FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473553
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69590.4722599051408398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT68110.46221615374540315No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCT51630.35037762469351286No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT45740.3104062086670788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45340.3076916812629067No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA43410.2945940865377764No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG41950.2846860615125482No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT40600.2755245315234674No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40150.2724706881937738No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA39980.27131701404700065No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG39290.2666344542748038No Hit
TTATAATAGTTATCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT38730.2628341159089629No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35610.24166080215642055No Hit
CTATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC34010.2308026925397322No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCG33590.22795243876535148No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC32720.2220483416612772No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA31210.21180100071052757No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG30690.20827211508510382No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGG29970.20338596575759404No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA29670.201350070204465No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC28640.19436016213872184No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGA28620.19422443576851325No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28270.19184922428986267No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28170.19117059243881965No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC28160.19110272925371535No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC28140.19096700288350674No Hit
GTGCAGCCACAGTTCGTTTGCTTTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGATAACTATTATAATGTTGGC27700.18798102273891742No Hit
CATTATAATAGTTATCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT27620.18743811725808301No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACATTATAATAGTTAT26670.18099111467317427No Hit
TCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGG26650.1808553883029657No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAA26530.18004103008171407No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT26420.17929453504556672No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24720.16775779357783535No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23640.16042856958657067No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGA23640.16042856958657067No Hit
GTGTATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC22990.15601746255479104No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22520.15282789285488882No Hit
ATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCC22260.15106345004217697No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21820.1480774698975877No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC21230.14407354197643385No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT21160.1435984996807037No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20460.13884807672340255No Hit
GTCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACATTATAATAGTTATCCCTGGACGTTCGGCCAAGGG20130.13660859161496058No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19970.13552278065329174No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19960.1354549174681874No Hit
TCTATAAGGCGTCTAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGTATCTGGGACAGAATTCACT19720.13382620102568418No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC19590.13294397961932825No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTG19420.13179030547255513No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19190.13022945221515617No Hit
GTCCAGGGATAACTATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGACTGCTGATGGTGAG18790.12751492481098406No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT18380.12473253422170766No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18330.12439321829618614No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA18090.1227645018536829No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18020.1222894595579528No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT17680.11998211126440651No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC17660.11984638489419791No Hit
CACCATCAGCAGTCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACATTATAATAGTTATCCCTGGACGTT17300.11740331023044302No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC17200.1167246783794No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTACAAAGTGGGGTCCCAT17070.11584245697304407No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA17050.11570673060283547No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17030.11557100423262685No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATT17020.11550314104752256No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16990.11529955149220965No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC16540.11224570816251606No Hit
ACCTTGATGGGACCCCACTTTGTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG16160.10966690712855254No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC16060.10898827527750952No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16050.10892041209240523No Hit
GGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA16010.10864895935198801No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15970.1083775066115708No Hit
ACTTATTACTGCCAACATTATAATAGTTATCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGA15760.10695237972438046No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15690.10647733742865034No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15610.10593443194781592No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG15530.10539152646698151No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATG15520.10532366328187721No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15480.10505221054146No Hit
GAGTGAATTCTGTCCCAGATACACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAACTAGACGCCTTAT15450.10484862098614708No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15170.10294845180322662No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA15010.1018626408415578No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14880.10098041943520186No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14800.10043751395436744No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC14780.10030178758415884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)