FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004717786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473553
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68680.46608435529634834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT59340.40270014040893No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCT46310.31427441021802405No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45060.3057915120799863No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA44070.29907305675466034No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT41810.283735976921088No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41520.28176794455306325No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT38910.26405565324084035No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG38100.2585587352473918No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37550.2548262600666552No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36380.24688626740945185No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA35340.2398284961586044No Hit
CTATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTC34220.23222781942692253No Hit
TTATAATAGTTATCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT34180.23195636668650535No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG30020.2037252816831156No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGG29570.20067143835342197No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC29280.1987034059853972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29100.19748186865351977No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG29020.19693896317268533No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC28760.19517452035997346No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28640.19436016213872184No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCG28140.19096700288350674No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27810.18872751777506477No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGA26670.18099111467317427No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26480.17970171415619254No Hit
GTGCAGCCACAGTTCGTTTGCTTTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGATAACTATTATAATGTTGGC26410.17922667186046243No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA26370.17895521912004522No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC26100.17712291312222905No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACATTATAATAGTTAT24970.1694543732054429No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC24960.16938651002033858No Hit
CATTATAATAGTTATCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCAT24920.16911505727992138No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAGAA24250.16456822387793313No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23820.16165010691844814No Hit
TCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGAACTGTGG23260.1578497685526072No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23210.15751045262708568No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT23150.15710327351645986No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC23060.15649250485052116No Hit
GAGTGAATTCTGTCCCAGATACACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAACTAGACGCCTTAT21930.14882396493373498No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGA21740.14753456441675325No Hit
GTGTATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC21530.1461094375295629No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT21280.14441285790195535No Hit
ATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCC21080.1430555941998693No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT20860.14156260412757465No Hit
GTCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACATTATAATAGTTATCCCTGGACGTTCGGCCAAGGG20280.1376265393915251No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20040.13599782294902185No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC19730.1338940642107885No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTAATACTCTG18560.1259540715535851No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC18460.12527543970254207No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC18070.12262877548347431No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT17880.12133937496649255No Hit
TCTATAAGGCGTCTAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGTATCTGGGACAGAATTCACT17820.12093219585586673No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC17730.12032142718992803No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17700.12011783763461512No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17620.11957493215378069No Hit
GTCCAGGGATAACTATTATAATGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGACTGCTGATGGTGAG17550.11909988985805058No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17310.11747117341554732No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG17180.11658895200919138No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16930.11489237238158383No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGGTACTA16920.11482450919647953No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16780.11387442460501929No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGATT16610.11272075045824616No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16460.11170280268168163No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16340.11088844446042999No Hit
CACCATCAGCAGTCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACATTATAATAGTTATCCCTGGACGTT16260.11034553897959559No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC16130.10946331757323965No Hit
ACTTATTACTGCCAACATTATAATAGTTATCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAAGCAAACGA16100.10925972801792674No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC16050.10892041209240523No Hit
CTGCTGATGGTGAGAGTGAATTCTGTCCCAGATACACTGCCGCTGAACCTTGATGGGACCCCACTTTGTAAACT15960.1083096434264665No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGCTTTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGATA15940.1081739170562579No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15920.1080381906860493No Hit
ACCTTGATGGGACCCCACTTTGTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG15300.10383067320958254No Hit
GGACCAAGGTGGAAAGCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA15180.10301631498833093No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA15140.10274486224791371No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15050.10213409358197499No Hit
GGCTGCAGACTGCTGATGGTGAGAGTGAATTCTGTCCCAGATACACTGCC15010.1018626408415578No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14950.10145546173093198No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGG14950.10145546173093198No Hit
GTATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAGCCTGATGA14900.10111614580541047No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTACAAAGTGGGGTCCCAT14770.10023392439905453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT859.931682E-997.9645270
CTCGGTT600.002301972869.3915470
TACGTAT1105.260781E-663.08321470
GTAGTTT1853.585228E-852.51251670
CAGTTCG28300.048.05915570
GTGGTAT2950.047.8190651
TCTAGTG6100.043.22751670
GATGTAT1309.095436E-442.70248870
ATTCCGG5550.042.5101370
GACTGGG22750.036.6021370
GTATCAA33700.036.17851
TACATTG6700.035.21361570
GTCCTTG41700.033.61412470
ATTAGGC601.6318667E-533.5313533
TATCAAC36350.033.2276652
TGAGCGG6300.033.0435970
ATCAACG36800.032.893533
TCAACGC37050.032.5810744
CAACGCA37600.031.9272145
ACATGTT4807.2759576E-1131.80445570
Produced by FastQC (version 0.11.5)