FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004717789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530090
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT57060.3729192400447032No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53780.35148259252723696No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAGGATT41230.26946127351985827No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37370.24423399930723028No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT36280.23711023534563327No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG35960.2350188551000268No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG35700.23331960865047152No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCG34780.22730689044435295No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA33750.22057526027880714No Hit
CTCTTGCAGGGCCAGCCAGGGTCTTTTTAATAATCACTTAGCCTGGTATC31000.20260246129312656No Hit
GTCCCAGACCCACCGCCACTGATCCTGTCTGGGATGCCGGTGACCCTGTT29530.19299518328987184No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29530.19299518328987184No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29140.19044631361553896No Hit
CATCTATGGTGCATCGAACAGGGTCACCGGCATCCCAGACAGGATCAGTGGCGGTGGGTCTGGGACAGACTTC28640.18717853198177886No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTT26330.17208138083380717No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT26300.17188531393578158No Hit
CTCCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTTTATTACTGTCACC26180.17110104634367912No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA25660.16770255344456864No Hit
GTATAAGGGGAGTAACCATGCTGGTGACAGTAATAAACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGAGAG25200.1646961943415093No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG24370.15927167682946752No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC24360.15920632119679234No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23250.1519518459698449No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGCCAGGGTCTTTTTAATA22530.14724624041723036No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTGATTATTAAAAAGACCCTGG22200.1450895045389487No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC21810.14254063486461582No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21480.14038389898633413No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21210.13861929690410368No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21180.13842323000607806No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC21120.13803109621002688No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20550.13430582514754036No Hit
GACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTTTATTACTGTCACCAGCATGGTTACTCC20030.13090733224842985No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19210.12554817036906327No Hit
GTAATAGACGGCCGTGTCGTCAGATGTCAGCCTCCTCAACTCCATATAGGCTGTGCTGGTGGACGTGTCAGTGGT18960.12391427955218322No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC18860.12326072322543119No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCCTGGCCAAAAGTATAAGGGGAGTAACCATGCTGGTGAC18450.1205811422857479No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18170.11875118457084224No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGCCAGG18150.11862047330549183No Hit
GTTTATTACTGTCACCAGCATGGTTACTCCCCTTATACTTTTGGCCAGGGGACCAGGGTGGAGATCAAACGA18120.11842440640746624No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT18020.11777085008071421No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17790.11626767052918456No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC17660.11541804730440693No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT17050.11143135371121961No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17010.11116993118051878No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT16920.11058173048644197No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGATCCTGTCTGGGATGCCGGTGACCCTGTTCGATGCACCAT16780.10966675162898915No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC16580.1083596389754851No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16430.10737930448535707No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC16330.10672574815860504No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16220.10600683619917783No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15990.10450365664764819No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15860.10365403342287055No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15610.10202014260599049No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15530.10149729754458889No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCCTGGCCAAAAGTATAAGGGGAGTA15480.10117051938121288No Hit
GTCTATTACTGTGCGAGAGGTTTTGGGGGAGCTCCGATTGACTTCTGGGGCCAGGGAACTCTGGTCCCCGTCTC15330.10019018489108485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)