FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530090
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53520.3497833460776817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52140.3407642687685038No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT48410.3163866177806534No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAGGATT39800.2601154180473044No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA35920.234757432569326No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35440.23162036220091628No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT33790.22083668280950794No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG32050.20946480272402276No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGCCG32020.20926873582599717No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30120.19685116561770877No Hit
CTCTTGCAGGGCCAGCCAGGGTCTTTTTAATAATCACTTAGCCTGGTATC27450.1794012116934298No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26080.1704474900169271No Hit
CATCTATGGTGCATCGAACAGGGTCACCGGCATCCCAGACAGGATCAGTGGCGGTGGGTCTGGGACAGACTTC25650.16763719781189343No Hit
GTCCCAGACCCACCGCCACTGATCCTGTCTGGGATGCCGGTGACCCTGTT25590.1672450640158422No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG25340.16561117319896215No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTT25190.1646308387088341No Hit
GTATAAGGGGAGTAACCATGCTGGTGACAGTAATAAACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGAGAG24680.1612977014423988No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC24180.1580299198086387No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG23350.15260540229659694No Hit
CTCCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTTTATTACTGTCACC23220.1517557790718193No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC22950.14999117698958883No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22100.14443594821219666No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21820.142605990497291No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21770.142279212333915No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC21130.13809645184270206No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA21020.13737753988327484No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21010.13731218425059963No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGCCAGGGTCTTTTTAATA20490.13391369135148912No Hit
GTAATAGACGGCCGTGTCGTCAGATGTCAGCCTCCTCAACTCCATATAGGCTGTGCTGGTGGACGTGTCAGTGGT20390.1332601350247371No Hit
GACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAGGATTTTGCAGTTTATTACTGTCACCAGCATGGTTACTCC20050.13103804351378023No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGATACCAGGCTAAGTGATTATTAAAAAGACCCTGG19000.12417570208288402No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18920.12365285702148239No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18860.12326072322543119No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC18740.12247645563332875No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG18620.12169218804122635No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCCTGGCCAAAAGTATAAGGGGAGTAACCATGCTGGTGAC18520.1210386317144743No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGCCAGG18230.11914331836689344No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT17920.1171172937539622No Hit
GTTTATTACTGTCACCAGCATGGTTACTCCCCTTATACTTTTGGCCAGGGGACCAGGGTGGAGATCAAACGA17600.11502591350835573No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17520.1145030684469541No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC17310.11313060016077485No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17090.11169277624192041No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC17070.11156206497657001No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT16910.11051637485376678No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGATCCTGTCTGGGATGCCGGTGACCCTGTTCGATGCACCAT16570.10829428334280991No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC16430.10737930448535707No Hit
GTCTATTACTGTGCGAGAGGTTTTGGGGGAGCTCCGATTGACTTCTGGGGCCAGGGAACTCTGGTCCCCGTCTC15780.10313118836146892No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGC15690.10254298766739212No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15500.10130123064656328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)