FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004717791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109491
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT43630.3932433881843116No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40830.36800659040947603No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT29100.26228243401704027No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG28770.25930809713643466No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCGCAGAGTTCAACTTCAGGGACA28550.2573252058826976No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG27180.24497720125715305No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26750.24110155017030332No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC23410.21099765568174958No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22970.20703187317427543No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA22790.20540950760303597No Hit
GGCCTGGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC22710.20468845623804066No Hit
GTCTGGGACAGACTTCACTCTCACCATGAGCGGCCTGCAGTCTGAGGATT22180.1999114909449468No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21370.1926108458743694No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21160.19071808604125676No Hit
TCCCAGGCCAGTTATCATATTGCTGACAGTAATAAACTGCAAAATCCTCA20730.18684243495440703No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC20600.18567072648628966No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA20430.18413849233567464No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18810.16953720219451984No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17690.15944248308458564No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17470.15745959183084857No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT17270.1556569634183603No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC17180.1548457806327406No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17160.15466551779149176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17010.15331354648212558No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16380.14763526698278762No Hit
CACCATGAGCGGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAGCAATATGATAACTGGCCTGGGACGTT16330.14718460987966556No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16270.14664382135591908No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATGAGCGGCCTGCAGTCTG15880.14312869595156696No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15740.1418668560628252No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTATCAGCAGAAACCTGGGCAGGCTCCCAGGCT15390.13871225634097079No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15230.1372701536109802No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15110.13618857656348723No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14890.13420568530975013No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC14710.13258331973851073No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCCCAGGCCAGTTATCATATTGCTGAC14690.1324030568972619No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCG14220.12816688012791452No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14210.1280767487072901No Hit
GTTTATTACTGTCAGCAATATGATAACTGGCCTGGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA14140.1274458287629192No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT14080.12690504023917273No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13720.12366030909669389No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13390.12068597221608827No Hit
GACTTCACTCTCACCATGAGCGGCCTGCAGTCTGAGGATTTTGCAGTTTATTACTGTCAGCAATATGATAACTGG13360.12041557795421504No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13320.1200550522717174No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13200.11897347522422444No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTCCCAGGCCAGTT13120.11825242385922914No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12890.11617940118486766No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12710.11455703561362825No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT12570.11329519572488644No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12550.11311493288363762No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12450.1122136186773935No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12290.11077151594740291No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12260.11050112168552967No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC12070.10878862469366583No Hit
GTAATAAACTGCAAAATCCTCAGACTGCAGGCCGCTCATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC11870.10698599628117758No Hit
CTCCCAGGCTCCTCATCTTTGGTGCATCCACCAGGGCCACTGGTATCCCA11830.10662547059867994No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG11640.1049129736068161No Hit
CTCATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGC11580.10437218508306963No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11570.10428205366244522No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11540.10401165940057198No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG11470.1033807394562011No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11460.10329060803557669No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11370.10247942524995696No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCGCAGAGTTCAACTTCAGGGACAATTGATTCTTGGACGTGTCTT11360.10238929382933254No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11340.10220903098808372No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGATGCACCAA11310.10193863672621048No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11150.10049653399621988No Hit
GTCTCTGGTTACTTCATCAGTAGTGGGTACTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTGG11130.10031627115497106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGAT9300.088.982470
GTTACGG150.002515408267.8665531
CTTATAC2050.039.5064931
GTATCAA33900.038.6227461
CAGTTCG16950.037.55553470
TTATACA2250.035.988322
TATACAC2500.035.0886083
TATCAAC37800.034.542362
CTTGTAT601.5625425E-533.7436116
TCTTGTT2651.1848315E-633.6299270
TCTCGCG11850.033.3055870
GCAATAT19850.032.7102170
ATCAACG41400.031.6201693
TCAACGC41600.031.3870494
GTGGCCG11400.031.26992470
CAGCCAT9900.030.86382170
CGTATGC2350.030.63440741
AACGCAG42650.030.6143286
CAACGCA42800.030.5070385
ACAGCTT2103.970122E-430.31268170
Produced by FastQC (version 0.11.5)