FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325461
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGGAGTGTTTGTTGTTTGTTTCATAGATCAGTGTCCTGGGGGCTTG52270.39435336083068456No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG49720.37511477138897337No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCGCCACTATAGGTGAGCAAGCAGTAATACTC49160.370889826256676No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48630.3668912174707517No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTGCGCAGC48000.36213815419691714No Hit
CCTATAGTGGCGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA46360.3497651005951891No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC45240.3413152103305944No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTGTGGTGACTCAGG45140.3405607558426842No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43750.3300738384607318No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA42730.32237840268404727No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41980.31671999402472045No Hit
GAGTATTACTGCTTGCTCACCTATAGTGGCGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC41400.31234415799484105No Hit
GTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTG41090.3100053490823193No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT39270.29627427740235285No Hit
GCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCACCTATAGTGGCGCTCGGGTGTTCGGCGGAGGGACCA36930.2786200423852531No Hit
CAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC34940.26360639807583924No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34210.2580988803140945No Hit
GTATTACTGCTTGCTCACCTATAGTGGCGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC34200.2580234348653035No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33880.25560918050399073No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33090.24964899004949978No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32150.2425571178631435No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT30940.23342821855942952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC29560.22301674662626816No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG29070.21931991963550795No Hit
CTATAGGTGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCC27640.20853122045839145No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCA27540.2077767659704812No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG27260.20566429340433254No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27100.20445716622367613No Hit
CCACTATAGGTGAGCAAGCAGTAATACTCAGCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTG26790.20211835731115435No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC26240.19796885762764804No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACCCTTTCGGGTG26230.197893412178857No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC25440.19193322172436608No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC25200.19012253095338152No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25200.19012253095338152No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25150.18974530370942638No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGG25030.1888399583239341No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24570.18536946767954696No Hit
GGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTTGTTTGTTTCAT23530.17752314100528044No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23260.17548611388792276No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA22930.17299641407781896No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22830.17224195958990873No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22610.1705821597165062No Hit
GTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC22450.1693750325358498No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCGCCACTATAGGTGAGCAAGCAGTAATACTCAGC22200.16748889631607417No Hit
CACCTATAGTGGCGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC21730.16394296022289603No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG21560.1626603875934486No Hit
GCACTGGAGCTGTCACCAGTGGTCATCATCCCAACTGGTTCCAGCAGAAG21490.16213226945191145No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT21080.15903900605147944No Hit
CCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCG20710.15624752444621154No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA20470.15443683367522698No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG20140.15194713386512315No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATC19810.14945743405501935No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC18890.1425164527662451No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18760.1415356619319618No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18470.13934774391702207No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT17870.13482101698956062No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17760.13399111705285935No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCCCAGGCTGTGGTGACTCAGGAGCCCTCACTG17650.13316121711615808No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17540.13233131717945681No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG17520.13218042628187476No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC17070.12878538108627866No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTTGTTTGTTTCATAGATCAGTGTCCTGGGGGCTTGGCCAGG17020.12840815384232354No Hit
AAGCTGCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTCACCTATAGTGGCG16890.12742736300804022No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16680.1258430085834287No Hit
CTCTCACCTGTGGCTCCAGCACTGGAGCTGTCACCAGTGGTCATCATCCCAACTGGTTCCAGCAGAAGCCTGGCC16420.12388142691486206No Hit
TCCCAACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATCT15580.11754400921641603No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC15520.11709133652366988No Hit
GCCTCATCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGC15410.11626143658696861No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15370.1159596547918045No Hit
GCCCTGACCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTG15330.1156578729966404No Hit
GTCCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTG15210.11475252761114813No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC15200.11467708216235709No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA15030.11339450953290968No Hit
GGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGTTGTTTGTTTC14980.11301728228895457No Hit
GGATGAGGCTGAGTATTACTGCTTGCTCACCTATAGTGGCGCTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGT14810.11173470965950714No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14790.1115838187619251No Hit
GTCCTGGGGGCTTGGCCAGGCTTCTGCTGGAACCAGTTGGGATGATGACCACTGGTGACAGCTCCAGTGCTGG14780.11150837331313408No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14760.11135748241555203No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG14460.10909411895182129No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGAGCGCC14310.10796243721995594No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG14230.10735887362962773No Hit
GTCACCAGTGGTCATCATCCCAACTGGTTCCAGCAGAAGCCTGGCCAAGCCCCCAGGACACTGATCTATGAAACA13870.10464283747315085No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13830.10434105567798675No Hit
CCCTTTCGGGTGCGCAGCCTGAGGATGAGGCTGAGTATTACTGCTTGCTC13830.10434105567798675No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13820.10426561022919573No Hit
GATCTATGAAACAAACAACAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC13700.10336026484370343No Hit
CCCCAGGAGGGACAGTCACTCTCACCTGTGGCTCCAGCACTGGAGCTGTC13470.10162501952150986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)