FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619620
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27690.44688680158807015No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT26200.42283980504179985No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18870.3045414931732352No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC17940.289532293986637No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG17480.28210838901262064No Hit
CTCCTAATCTATGAAAGGTCCAATTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGAC17150.27678254413995673No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16140.2604822310448339No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT15970.2577386139892192No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG15630.25225137987798973No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15350.24773248119815372No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA14320.23110938962589972No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCATTCTCACCATCAGCAGCCTGCAGCCTG13570.21900519673348184No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13440.21690713663212938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13300.21464768729221134No Hit
GATTAGGAGTGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGGCTAACCTGCCAACACCCTGGCTCGCCCGACA13170.2125496271908589No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC13020.21012878861237533No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA12910.20835350698815402No Hit
ACTGTCAACAGGGTGACAGGTTCCCGCTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG12460.20109099125270327No Hit
CAGTAATATCGACCCGTGTCCTCACTTCTCAGACTGCTTATTTGAAGATA12360.19947709886704756No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12250.19770181724282623No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACA12130.1957651463800394No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11880.19173041541590008No Hit
GTGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGGCTAACCTGCCAACA11690.1886640198831542No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT11560.18656595978180174No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11560.18656595978180174No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC11470.1851134566347116No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC11370.18349956424905586No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11250.181562893386269No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCACCGCCGAAAGTGAGCGGGAACCTGTCACCCTGTTGAC10840.17494593460508054No Hit
AGTTACTACTGTCAACAGGGTGACAGGTTCCCGCTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGA10760.17365482069655597No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10300.16623091572253962No Hit
AGCAAATACTACGCAGACTCCGTTAAGGGCAGATTCGCCATCTCCAGAGACAATTCCAAGAACACGTTGTATCTT10280.16590813724540848No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10160.1639714663826216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10140.16364868790549045No Hit
ATATTACTGTGTGAAAGACCATTGCCCCAGTACCAGCTGCTATCGTCTTGACTATTGGGGCCAGGGAACCCTGGT10130.16348729866692488No Hit
GACTTCATTCTCACCATCAGCAGCCTGCAGCCTGAGGACTTTGCAAGTTACTACTGTCAACAGGGTGACAGGTTC10070.16251896323553144No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10030.16187340628126917No Hit
ATCTGGGACAGACTTCATTCTCACCATCAGCAGCCTGCAGCCTGAGGACT9870.15929117846422No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCACCGCCGAAAGTGAGCGGGAAC9550.1541267228301217No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTG9330.15057615958167908No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGCCAGGGTGTTGGCAG9250.1492850456731545No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9050.14605726090184307No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATG8810.14218391917626932No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8780.1416997514605726No Hit
GTTGACAGTAGTAACTTGCAAAGTCCTCAGGCTGCAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGATCC8780.1416997514605726No Hit
CCCCTACACTCCTAATCTATGAAAGGTCCAATTTGCACAGTGGGGTCCCA8740.14105419450631032No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC8690.14024724831348245No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8690.14024724831348245No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8580.13847196668926115No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8570.13831057745069558No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC8490.137019463542171No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT8470.13669668506503987No Hit
GGGTGACAGGTTCCCGCTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT8380.1352441819179497No Hit
GGACCCCACTGTGCAAATTGGACCTTTCATAGATTAGGAGTGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGG8370.13508279267938414No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8110.13088667247667926No Hit
GTCCTCAGGCTGCAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC8060.1300797262838514No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGC7990.1289500016138924No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7980.1287886123753268No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC7960.12846583389819566No Hit
GTAGTAACTTGCAAAGTCCTCAGGCTGCAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGATCCACTGCCG7890.12733610922823665No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG7860.12685194151253995No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTT7760.12523804912688422No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC7560.12201026435557276No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA7450.12023498273135147No Hit
AGCCAGGGTGTTGGCAGGTTAGCCTGGTATCAGCAGAAACCGGGGAAAGC7410.11958942577708917No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC7400.11942803653852362No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT7360.11878247958426133No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA7310.11797553339143345No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7290.11765275491430231No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC7210.11636164100577774No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACC7190.11603886252864659No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCC7100.11458635938155644No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT7100.11458635938155644No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT6980.11264968851876958No Hit
CTCCTGTTCAGCCTCTGGATTCACATTTAGTGACTATGCTATTCACTGGGTCCGCCAGGCTCCAGGGAAGGGC6770.10926051450889254No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6730.10861495755463024No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6540.10554856202188438No Hit
GTGTTGGCAGGTTAGCCTGGTATCAGCAGAAACCGGGGAAAGCCCCTACA6520.10522578354475323No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC6500.10490300506762208No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT6430.10377328039766309No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC6410.10345050192053194No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGGGGAAGACCGATGGGCCCTTGGTGG6370.10280494496626964No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6360.10264355572770407No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC6340.10232077725057293No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC6310.10183660953487622No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6280.1013524418191795No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA6260.10102966334204835No Hit
GCCTGAGGACTTTGCAAGTTACTACTGTCAACAGGGTGACAGGTTCCCGC6240.1007068848649172No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6210.10022271714922049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)