FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619620
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27230.43946289661405374No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT22090.35650882799134953No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17620.28436783835253865No Hit
CTCCTAATCTATGAAAGGTCCAATTTGCACAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGAC17130.2764597656628256No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA16520.2666150221103257No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGACTTCATTCTCACCATCAGCAGCCTGCAGCCTG16310.26322584810044863No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC16100.25983667409057165No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14970.24159969013266194No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14900.24046996546270294No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT14820.23917885155417834No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG14740.2378877376456538No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14620.2359510667828669No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12940.20883767470385073No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC12720.20528711145540815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12530.20222071592266225No Hit
GATTAGGAGTGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGGCTAACCTGCCAACACCCTGGCTCGCCCGACA12290.19834737419708853No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG11800.19043930150737548No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11610.18737290597462963No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11560.18656595978180174No Hit
ACTGTCAACAGGGTGACAGGTTCCCGCTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG11540.1862431813046706No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC11480.1852748458732772No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11420.18430651044188373No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCACCGCCGAAAGTGAGCGGGAACCTGTCACCCTGTTGAC11390.18382234272618703No Hit
GTGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGGCTAACCTGCCAACA11300.18236983957909686No Hit
CAGTAATATCGACCCGTGTCCTCACTTCTCAGACTGCTTATTTGAAGATA11100.17914205480778542No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10970.17704399470643298No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC10570.17058842516381006No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10450.1686517543010232No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC9870.15929117846422No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGCCAGGGTGTTGGCAG9860.15912978922565443No Hit
ATATTACTGTGTGAAAGACCATTGCCCCAGTACCAGCTGCTATCGTCTTGACTATTGGGGCCAGGGAACCCTGGT9700.1565475614086053No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT9680.15622478293147413No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACA9600.15493366902294956No Hit
GGGTGACAGGTTCCCGCTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9580.15461089054581842No Hit
GTTGACAGTAGTAACTTGCAAAGTCCTCAGGCTGCAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGATCC9320.1504147703431135No Hit
AGCAAATACTACGCAGACTCCGTTAAGGGCAGATTCGCCATCTCCAGAGACAATTCCAAGAACACGTTGTATCTT9310.15025338110454795No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA9280.14976921338885124No Hit
GTCCTCAGGCTGCAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC9220.1488008779574578No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9170.14799393176462994No Hit
GACTTCATTCTCACCATCAGCAGCCTGCAGCCTGAGGACTTTGCAAGTTACTACTGTCAACAGGGTGACAGGTTC9150.1476711532874988No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCACCGCCGAAAGTGAGCGGGAAC9080.14654142861753977No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8990.14508892547044963No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTGTGCAAATTGGACCTTTCATAGATTAGGAGTGT8980.14492753623188406No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTG8970.1447661469933185No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC8970.1447661469933185No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8920.14395920080049063No Hit
AGTTACTACTGTCAACAGGGTGACAGGTTCCCGCTCACTTTCGGCGGTGGGACCAAGGTGGAGATCAAACGA8910.14379781156192503No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8640.13944030212065459No Hit
ATCTGGGACAGACTTCATTCTCACCATCAGCAGCCTGCAGCCTGAGGACT8600.1387947451663923No Hit
GTAGTAACTTGCAAAGTCCTCAGGCTGCAGGCTGCTGATGGTGAGAATGAAGTCTGTCCCAGATCCACTGCCG8560.13814918821213001No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATG8420.135889738872212No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8380.1352441819179497No Hit
GAATGAAGTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTGTGCAAATTGGACCTTTCAT8200.1323391756237694No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC7990.1289500016138924No Hit
CCCCTACACTCCTAATCTATGAAAGGTCCAATTTGCACAGTGGGGTCCCA7980.1287886123753268No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT7960.12846583389819566No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT7950.1283044446596301No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACC7950.1283044446596301No Hit
GGACCCCACTGTGCAAATTGGACCTTTCATAGATTAGGAGTGTAGGGGCTTTCCCCGGTTTCTGCTGATACCAGG7670.12378554597979408No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT7530.12152609663987607No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC7460.12039637196991704No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7460.12039637196991704No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC7380.11910525806139247No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATT7330.1182983118685646No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7040.113618023950163No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA7000.1129724669959007No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG6880.11103579613311383No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6870.11087440689454826No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6840.11039023917885156No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6730.10861495755463024No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC6580.10619411897614668No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC6570.1060327297375811No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC6560.10587134049901553No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG6500.10490300506762208No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT6480.10458022659049093No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6440.10393466963622866No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6400.10328911268196637No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTT6360.10264355572770407No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC6330.10215938801200736No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGC6270.10119105258061392No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT6230.10054549562635164No Hit
CTCCTGTTCAGCCTCTGGATTCACATTTAGTGACTATGCTATTCACTGGGTCCGCCAGGCTCCAGGGAAGGGC6220.10038410638778605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)