FastQCFastQC Report
Wed 8 May 2024
EGAF00004717799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854572
Total Bases62.7 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49720.5818117139339927No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT48450.5669504734533778No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34800.40722139269716306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33620.393413310990765No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31710.3710629414490529No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31530.36895662390061923No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30260.3540953834200044No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29970.3507018718141947No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28660.3353725607672613No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28410.33244711972777014No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT28060.32835150227248255No Hit
GGGTATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCTGGAAGAGCCC27830.32566009651615074No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27420.32086237321138533No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26130.3057670974476112No Hit
GTCTCCATCCTTCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTT25370.2968737566875582No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA25360.29675673904597855No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24550.2872783100780274No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24190.28306567498116014No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG23900.2796721633753505No Hit
ACTTATTAGTTACCCAATCACTTTCGGCCCTGGGACCAAAGTGGATATCA23550.2755765459200629No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23390.2737042636547886No Hit
GTATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCTGGAAGAGCCCCT23280.2724170695974125No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC22060.25814091732469585No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC21600.2527581058120322No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21590.25264108817045255No Hit
CTAATAAGTTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCT21530.2519389823209747No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA20760.24292862391934208No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT20400.23871598882247488No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19280.22561001296555466No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGTATTAGCAC19040.22280158956764323No Hit
TATTTAGCCTGGTATCAGCAAAAACCTGGAAGAGCCCCTAACCTCCTGAT18580.21741877805497956No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGA18470.2161315839976035No Hit
GTAATAGACAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGAAACA18270.21379123116601056No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC18010.21074877248493984No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17980.2103977195602009No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT17030.19928104361013466No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA16920.19799384955275856No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG16730.19577051436274534No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC16550.19366419681431174No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16460.1926110380400949No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA16430.192259985115356No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16340.1912068263411392No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCC16320.1909727910579799No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15460.18090927388213046No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15440.18067523859897117No Hit
GTGCTAATACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTAC14970.1751754094447279No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14700.1720159331220775No Hit
CTGTTGACCCAGTCTCCATCCTTCCTGTCTGCTTCTGTAGGAGACAGAGT14690.17189891548049785No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCAAAGATCAGGAGG14590.1707287390647014No Hit
GATCAGGAGGTTAGGGGCTCTTCCAGGTTTTTGCTGATACCAGGCTAAAT14390.1683883862331085No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14280.1671011921757324No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14270.16698417453415276No Hit
CTAATACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA14000.16382469821150236No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC13890.16253750415412627No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13840.16195241594622806No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13560.158675921981998No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA13540.1584418866988387No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13490.1578567984909405No Hit
GGTTAGGGGCTCTTCCAGGTTTTTGCTGATACCAGGCTAAATAAGTGCTA13370.15645258679198476No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA13310.1557504809425069No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13240.15493135745144937No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG12950.1515378458456397No Hit
GTATATGGTAGTCCCACTAGCACTAATATATGAAATCCACTCCAGCCCCT12780.14954854593878575No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12700.14861240480614857No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC12580.14720819310719285No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC12560.14697415782403356No Hit
GTCTATTACTGTGCGAGACTTTGGGTGGGAACTTATCCAGGGGAGAAAAA12480.1460380166913964No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC12400.14510187555875922No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12370.1447508226340203No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12290.14381468150138316No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12200.14276152272716636No Hit
GTCAGGGTATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCTGGAAGA12200.14276152272716636No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT12100.1415913463113699No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT12010.14053818753715308No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATC11980.14018713461241417No Hit
GCCCCTAACCTCCTGATCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCC11920.1394850287629363No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11880.13901695819661772No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11800.13808081706398057No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11640.13620853479870626No Hit
GGCTAAATAAGTGCTAATACCCTGACTGGCCCGGCAAGTGATGGTGACTC11340.13269800555131692No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11340.13269800555131692No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11300.13222993498499835No Hit
GCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATTGG11090.1297725645118258No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11030.12907045866234793No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11020.12895344102076828No Hit
GTCTCCTACAGAAGCAGACAGGAAGGATGGAGACTGGGTCAACAGGATGT10860.12708115875549397No Hit
TACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCA10720.12544291177337896No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10610.12415571771600287No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10580.12380466479126394No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10530.12321957658336571No Hit
CCCCTAACCTCCTGATCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCCA10230.11970904733597637No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA10090.11807080035386136No Hit
CCATCCTTCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG9990.1169006239380649No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCTGTTGACCCAGTC9890.11573044752226846No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGA9860.11537939459752952No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9770.11432623582331274No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9760.11420921818173309No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA9690.11339009469067557No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT9660.11303904176593663No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9630.1126879888411977No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCA9570.11198588299171984No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC9520.11140079478382162No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCTGTTGACCCAGTCTCCAT9490.11104974185908267No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCT9450.11058167129276408No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9370.10964553016012694No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG9360.1095285125185473No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9340.109294477235388No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9330.10917745959380835No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGGGTATTAGCACTTATTTAGCC9290.10870938902748978No Hit
CACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAAC9210.10777324789485263No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9110.10660307147905618No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGA9100.10648605383747653No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG8990.10519885978010045No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT8980.1050818421385208No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGTATTAGC8960.1048478068553615No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT8860.10367763043956507No Hit
CTTCCAGGTTTTTGCTGATACCAGGCTAAATAAGTGCTAATACCCTGACT8840.10344359515640578No Hit
CAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGT8840.10344359515640578No Hit
GGACCCCACTTTGCAAAGTGGATGCAGCAAAGATCAGGAGGTTAGGGGCT8840.10344359515640578No Hit
CTTTGGTCCCAGGGCCGAAAGTGATTGGGTAACTAATAAGTTGTTGACAA8760.10250745402376861No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA8690.10168833053271112No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8580.10040113647533504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)