FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854572
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43120.5045800704914273No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT38500.4505179200816315No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31320.3664992534274467No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA29880.3496487130399779No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG27040.31641570283135884No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT26280.3075223620713059No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC24690.28891655706014235No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23930.2800232163000894No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23660.27686373997743896No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23340.27311917544689035No Hit
GGGTATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCTGGAAGAGCCCCTAACCTCCTGATCTTTGCTGCATC22640.26492794053631524No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC22110.2587260055325941No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20710.24234353571144385No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC20350.23813090061457665No Hit
GTCTCCATCCTTCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGTATTAGC20110.2353224772166652No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20080.23497142429192625No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATTGGGTAACTAATAAGTTGTTGAC19730.2308758068366387No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATC19720.23075878919505904No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG19500.22818440108030688No Hit
ACTTATTAGTTACCCAATCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT19450.22759931287240864No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19020.22256755428448394No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG18490.21636561928076278No Hit
CTAATAAGTTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC18230.213323160599692No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC17950.21004666663546195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17900.20946157842756372No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17770.20794034908702838No Hit
GTATTAGCACTTATTTAGCCTGGTATCAGCAAAAACCTGGAAGAGCCCCTAACCTCCTGATCTTTGCTGCATCC17310.2025575375743647No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17110.20021718474277184No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT16740.195887532004325No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16440.19237700275693562No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16290.19062173813324096No Hit
GTAATAGACAGCCGTGTCGTCGTCTCTCAGGCTGTTCATTTGCAGAAACAGTGAATTCTTGTCGTTGTCTCTGG15640.18301559143056406No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC15630.18289857378898441No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGACAAGAATTCACTGTTTCTG15470.1810262915237101No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14590.1707287390647014No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACAACTTATTAGTTAC14490.16955856264890495No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT14440.16897347444100672No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14310.16745224510047135No Hit
GTGCTAATACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGAAGGATGG14310.16745224510047135No Hit
TATTTAGCCTGGTATCAGCAAAAACCTGGAAGAGCCCCTAACCTCCTGAT14140.1654629451936174No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC13860.16218645122938732No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCTGTTGACCCAGTCTCCATCCTTC13780.1612503100967502No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13620.15937802783147587No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGTATTAGCACTTATTTAGCCTGGTATCAGCAAAA13570.15879293962357766No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT13180.1542292516019715No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCC12910.1510697752793211No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTG12490.14615503433297605No Hit
GGTTAGGGGCTCTTCCAGGTTTTTGCTGATACCAGGCTAAATAAGTGCTA12480.1460380166913964No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT12460.1458039814082371No Hit
CTAATACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA12360.14463380499244066No Hit
GATCAGGAGGTTAGGGGCTCTTCCAGGTTTTTGCTGATACCAGGCTAAAT12100.1415913463113699No Hit
CTGTTGACCCAGTCTCCATCCTTCCTGTCTGCTTCTGTAGGAGACAGAGT12020.14065520517873273No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC11830.13843186998871948No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC11770.13772976413924165No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11650.13632555244028588No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCAAAGATCAGGAGGTTAGGGGCTCTTCCAGGTTTTTG11490.13445327017501157No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11440.13386818196711336No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11300.13222993498499835No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11170.13070870564446296No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG11090.1297725645118258No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCAAAGATCAGGAGGTT10960.12825133517129045No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10820.12661308818917538No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10800.1263790529060161No Hit
GTATATGGTAGTCCCACTAGCACTAATATATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT10690.12509185884864002No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10680.12497484120706037No Hit
GGCTAAATAAGTGCTAATACCCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGAAGCAGACAGGA10670.12485782356548072No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10610.12415571771600287No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10490.12275150601704712No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10480.12263448837546748No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA10330.12087922375177282No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10160.11888992384491885No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10020.11725167686280384No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCTGTTG9940.11631553573016669No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATTGGGTA9840.11514535931437023No Hit
GCCCCTAACCTCCTGATCTTTGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT9820.11491132403121095No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC9770.11432623582331274No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9760.11420921818173309No Hit
GTCTATTACTGTGCGAGACTTTGGGTGGGAACTTATCCAGGGGAGAAAAACTTTGACTACTGGGGCCAGGGAACC9680.11327307704909592No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG9670.11315605940751627No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9590.11221991827487914No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATTTTG9580.11210290063329949No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC9510.11128377714224197No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9490.11104974185908267No Hit
GTCTCCTACAGAAGCAGACAGGAAGGATGGAGACTGGGTCAACAGGATGTCACATCTGGCACCTGGGAGCCAG9010.10543289506325974No Hit
ATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAGATT8930.10449675393062258No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT8930.10449675393062258No Hit
CTCACAATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCA8800.1029755245900872No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8760.10250745402376861No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8650.10122025996639253No Hit
CCATCCTTCCTGTCTGCTTCTGTAGGAGACAGAGTCACCATCACTTGCCG8550.1000500835505961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)