FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004717804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1618243
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49610.30656706069484No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47110.2911182065981438No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC39480.2439683038950269No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTG37960.23457540060423557No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC37250.23018792604077384No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36930.22821047271639672No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC36580.22604763314285925No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35140.21714909318316222No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34060.21047518821338948No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC33450.20670566781379557No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32710.20213280700117348No Hit
GACCTAGGACGGTGACTGAGGTCCCAGTTCCGAAGACATAAGCACTCAGGCTACTGTCCCATGTTCCGCAGTAAT32110.1984250820179664No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCC31770.19632403786081573No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC30690.18965013289104293No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACATTTTTTACAGAGGTCAGACAAATGGT30210.18668395290447726No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT27690.17111150797500746No Hit
ACCTAGGACGGTGACTGAGGTCCCAGTTCCGAAGACATAAGCACTCAGGCTACTGTCCCATGTTCCGCAGTAATA26880.1661060792476779No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25540.1578254934518487No Hit
GCCTTGGGCTGACCTAGGACGGTGACTGAGGTCCCAGTTCCGAAGACATAAGCACTCAGGCTACTGTCCCATGTT25520.15770190261907513No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG24250.14985388473795344No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC24010.1483707947446706No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAA23660.14620795517113314No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22750.1405845722799357No Hit
GTCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCC22450.13873070978833216No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG21720.13421964439209688No Hit
ACATGGGACAGTAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCTCAGTCACCGTCCTAGGTCAGCCCAAGGCC21040.1300175560777955No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT20360.12581546776349412No Hit
GATTATTACTGCGGAACATGGGACAGTAGCCTGAGTGCTTATGTCTTCGGAACTGGGACCTCAGTCACCGTCCT19820.12247851527860773No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC19800.12235492444583415No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACTGAGGTCCCAGTTCCGAAGACATAAGCACTC19520.12062465278700417No Hit
ACTATGTATCGTGGTACCAGCAGTTCCCAGGAACAGCCCCCAAAGTCCTCATTTATGACAACAATAAGCGGCCCT19500.1205010619542306No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18790.11611358739076888No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGA18740.11580461030883495No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC18430.11388895240084461No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTG18260.11283843032226927No Hit
AGGCAGGACTCGGGACAATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTGCACAGGGT18160.11222047615840143No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCTGTGGTCCCACTCGTATATGGTGGTG18140.11209688532562785No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCAC17970.11104636324705251No Hit
CTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATAACTATGTATCGTGGTACCAGCAGTTCCCAGGAACAGCCCC17800.10999584116847716No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAG17590.10869813742435469No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGAATA17490.10808018326048685No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTTACAAAATGGATTGTTTCAAATGGCA17380.10740043368023221No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCT16930.10461963994282687No Hit
GGGTGGCTGACGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGAATCCCT16810.10387809494618547No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGAAGCTCTGGCTCTCCAAGGCAAAGTC16440.10159166453987442No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16360.10109730120878013No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACA16290.10066473329407265No Hit
GGGCATCACCGGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAA16210.10017036996297836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)