FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171974
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTC75050.6403725679921227No Hit
GTAATAATGAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAG67590.5767192787553307No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC55960.4774849954009218No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG52690.4495833525317115No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCACTCAGGCTGTCATCCCATGCTGCACAGT51450.43900291303390687No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC50470.43064095278564196No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48910.41733007728840404No Hit
GTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC41880.35734581142585076No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA40160.342669717928896No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37810.32261807855805674No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCC36680.31297622643505746No Hit
CCACAAGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATGAGCC36110.3081126373110666No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35880.3061501364364738No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35580.303590352687005No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33940.28959686818990865No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG31940.2725316431934497No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31610.26971588106903394No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC30830.26306044332041495No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT30550.2606713118209107No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC30170.2574289190715835No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT29240.24949358944823008No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC28780.24556858769904452No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28620.2442033696993278No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC27980.2387424977004609No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGC26980.23020988520223146No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26610.22705281857788653No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26370.22500499157831147No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26360.2249196654533292No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTC25970.22159194657901965No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA25560.2180935754547456No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG25220.21519248720534753No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA25180.2148511827054184No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCC23950.20435606933259612No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCT23750.2026495468329502No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC23650.2017962855831273No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23570.2011136765832689No Hit
GCATGGGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAA23270.19855389283380004No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG22950.19582345683436664No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG22910.19548215233443747No Hit
CCCCTGAGGGCCACTGACTATTCTGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGGTACCAGTAT22440.19147182446026959No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCACTCAGGCTGTCATCCCATGCTGCACAGTA21700.18515769121157977No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21550.18387779933684537No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21350.18217127683719947No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA20930.17858757958794308No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20790.17739301383819095No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20040.17099355446451883No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19710.1681777923401031No Hit
GTTGGTACCAGTATACATAATTATTTCCGATGTTGGAGCTGCTTCCAGAA19530.1666419220904218No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACA19260.16433811671589985No Hit
ATCCCATGCTGCACAGTAATAATGAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCC19200.16382615996600608No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18170.1550375690928297No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18150.1548669168428651No Hit
CAGTAATAATGAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC18090.15435496009297134No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTCATTATTACTGTGCAG18050.15401365559304217No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17970.1533310465931838No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17870.15247778534336087No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC17610.15025930609382118No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA17550.14974734934392742No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG17360.14812615296926382No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC17230.147016913344494No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT17080.14573702146975956No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16780.1431772377202907No Hit
GGTACCAACAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATCAGAATAGTCAGTGGCCCTCAGGGGTCCCTG16710.14257995484541466No Hit
GAATAGTCAGTGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCAT16540.14112941072071566No Hit
GTCCGAGGATGAGGCTCATTATTACTGTGCAGCATGGGATGACAGCCTGA16510.14087343234576874No Hit
ATGCTGCACAGTAATAATGAGCCTCATCCTCGGACCGGAGCCCACTGATG16500.14078810622078647No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16470.14053212784583957No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC16280.13891093147117597No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC16190.13814299634633534No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15980.13635114772170714No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT15610.13319408109736222No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15450.1318288630976455No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15390.13131690634775173No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15190.12961038384810586No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG15170.12943973159814126No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACC14940.1274772307235485No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC14890.127050600098637No Hit
CCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14860.12679462172369013No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG14780.12611201272383177No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14580.12440549022418586No Hit
GGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC14490.12363755509934521No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC14260.12167505422475242No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14150.12073646684994718No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14110.120395162350018No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCACTG13550.1156168993510095No Hit
GTCAGGGACCCCTGAGGGCCACTGACTATTCTGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGGT13400.11433700747627508No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC13380.11416635522631048No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13300.11348374622645212No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAG13240.11297178947655837No Hit
ATTCTGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGGTACC13150.11220385435171769No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC13080.11160657147684164No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAATTATGTATACTGGTACCAACAGCTCCCAGGAACGGCCCC13070.11152124535185934No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG12990.11083863635200099No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC12970.1106679841020364No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATCAGAATAGTCAGTGGCCCTCAGGGGTCCCTGACCGATTCTCTGG12860.10972939672723116No Hit
GCTTGGTCCCTCCGCCGAATACCACAAGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATGAGCCTCAT12760.1088761354774082No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGTTGGTACCAGTATACATAATTATTTCCGATGTTGGAGCTGCTTCCAGAAC12610.10759624360267377No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC12390.10571906885306329No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12390.10571906885306329No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG12370.10554841660309872No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12340.10529243822815183No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT12250.10452450310331116No Hit
CAGTAATACAGGGCTGTGTCCTCGGTTTTCAGGCCGCTCATTTGCAGAAA12190.10401254635341739No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12140.10358591572850592No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGG12120.10341526347854132No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12100.10324461122857675No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG11990.10230602385377151No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC11980.1022206977287892No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC11890.10145276260394856No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11840.10102613197903708No Hit
GTAATAATGAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC11820.10085547972907248No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTC11810.1007701536040902No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGCTCATTATTACTGTGCAGCATGG11790.1005995013541256No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG11720.10000221847924955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)