FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171974
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTC70720.6034263558747891No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52930.4516311795312865No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG47150.40231267929152015No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45700.38994039116908735No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCC44060.375946906671991No Hit
GTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC42400.3617827699249301No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA41230.3517996133020016No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCACTCAGGCTGTCATCCCATGCTGCACAGT40040.34164580442910847No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC38680.3300414514315164No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33570.28643980156556376No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT33070.282173495316449No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32630.27841914581722804No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTC31550.2692039243191402No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31360.2675827279444766No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC30410.25947674607115856No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGC30290.258452832571371No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCC30250.25811152807144183No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC30100.25683163619670746No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT30060.25649033169677826No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG29670.25316261282246877No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC28710.24497130482416846No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG27970.23865717157547864No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27430.23404956082643472No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC26910.2296126023273554No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25800.22014140245432065No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC25180.2148511827054184No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG25170.2147658565804361No Hit
GTAATAATGAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAG25130.21442455208050692No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG24900.21246205120591413No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCT24560.20956096295651608No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24260.20700117920704728No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23190.1978712838339417No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA22790.1944582388346499No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21770.18575497408645586No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC21580.18413377771179226No Hit
GCATGGGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAA21440.1829392119620401No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21410.1826832335870932No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21380.18242725521214634No Hit
CCCCTGAGGGCCACTGACTATTCTGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGGTACCAGTAT21210.18097671108744734No Hit
GTTGGTACCAGTATACATAATTATTTCCGATGTTGGAGCTGCTTCCAGAA21210.18097671108744734No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG20960.17884355796288998No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC20880.1781609489630316No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20830.17773431833812012No Hit
GAATAGTCAGTGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCAT20650.17619844808843882No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19620.16740985721526244No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA19530.1666419220904218No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19280.16450876896586444No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT18880.16109572396657265No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG18780.1602424627167497No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18520.15802398346721003No Hit
CCACAAGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATGAGCC18380.1568294177174579No Hit
CCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG17810.15196582859346708No Hit
GGTACCAACAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATCAGAATAGTCAGTGGCCCTCAGGGGTCCCTG17780.1517098502185202No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17600.1501739799688389No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17460.14897941421908678No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT17200.14676093496954712No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA17190.14667560884456482No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTCATTATTACTGTGCAG17180.14659028271958252No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC17060.145566369219795No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAATTATGTATACTGGTACCAACAGCTCCCAGGAACGGCCCC16960.14471310796997203No Hit
GTCCGAGGATGAGGCTCATTATTACTGTGCAGCATGGGATGACAGCCTGA16870.1439451728451314No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGC16740.14283593322036153No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG16660.1421533242205032No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC16230.1384843008462645No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC16180.13805767022135304No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15940.13600984322177795No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15610.13319408109736222No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACA15580.13293810272241535No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15310.1306342973478934No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15210.12978103609807043No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACCACTCAGGCTGTCATCCCATGCTGCACAGTA15200.12969570997308813No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC15080.1286717964733006No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14590.12449081634916817No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC14460.12338157672439833No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGG14410.12295494609948684No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14320.12218701097464621No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATCAGAATAGTCAGTGGCCCTCAGGGGTCCCTGACCGATTCTCTGG14190.12107777134987636No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGC14050.11988320560012425No Hit
GGTACCAACAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATCAGAATAGTCAGTGGCCCTCAGGGGTCC14020.11962722722517737No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCC13950.11902994435030129No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC13830.11800603085051375No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG13770.11749407410061997No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC13720.1170674434757085No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCACTG13520.11536092097606261No Hit
GCCCCCAAACTCCTCATCTATCAGAATAGTCAGTGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13500.11519026872609801No Hit
ATCAGAATAGTCAGTGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG13490.11510494260111573No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCAC13330.113739724601399No Hit
GGATGACAGCCTGAGTGGTCTTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC13320.11365439847641672No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG13270.11322776785150523No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13200.11263048497662917No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13110.11186254985178852No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGTTGGTACCAGTATACATAATTATTTCCGATGTTGGAGCTGCTTCCAGAAC13050.11135059310189475No Hit
CCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTCATTATTACTGTGCAGCATGGGATGAC12970.1106679841020364No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12940.11041200572708952No Hit
ATTCTGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGGTACC12900.11007070122716033No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG12850.10964407060224884No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGG12710.10844950485249673No Hit
GTCAGGGACCCCTGAGGGCCACTGACTATTCTGATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGGT12700.10836417872751444No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC12680.10819352647754985No Hit
GTATACTGGTACCAACAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTA12650.10793754810260296No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12640.10785222197762068No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGG12610.10759624360267377No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGC12570.1072549391027446No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGCTCATTATTACTGTGCAGCATGG12560.10716961297776231No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAATAATTAT12400.1058043949780456No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12380.105633742728081No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAAGACC12330.10520711210316952No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG12200.10409787247839969No Hit
ATGTATACTGGTACCAACAGCTCCCAGGAACGGCCCCCAAACTCCTCATC12160.10375656797847052No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12140.10358591572850592No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA11960.10205004547882461No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT11920.10170874097889543No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11800.10068482747910791No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTC11780.10051417522914331No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC11720.10000221847924955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)