FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519011
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATCTTAACTCACCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGT15100.2909379569989846No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13910.2680097338977401No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAACGTGTTGTTGGAATTGTCTCTGGA11290.21752910824626068No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC10600.20423459233041302No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA10220.19691297486951143No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCATACCCACTGCCA9740.18766461597153047No Hit
ATATATTACTGTGCGACAGACTCCGGTGATTCCCCTTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC8770.1689752240318606No Hit
GTATGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT8660.16685580845107328No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT8390.16165360657095898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7790.15009315794848277No Hit
GTGTATTACTGTCAGCAGTATCTTAACTCACCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA7790.15009315794848277No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7680.1479737423676955No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC7670.14778106822398754No Hit
ATCTTAACTCACCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA7320.14103747319420976No Hit
GTCCCATACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTTCTGGATGCACCATAGATGAGGAGCCTG7090.1366059678889272No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCTCCCACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAG7090.1366059678889272No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCATACCCACTGCCA6560.12639423827240656No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC6450.12427482269161925No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6390.12311877782937163No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6380.1229261036856637No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC6340.12215540711083195No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC6210.11965064324262877No Hit
GTCTCCAGGCACCCTGTCTTCGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCA6040.11637518279959386No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA5960.11483378964993035No Hit
GAGTTAAGATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGT5910.11387041893139067No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA5840.11252169992543509No Hit
CATCTATGGTGCATCCAGAAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTATGGGACAGACTTCA5720.11020961020093986No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC5560.10712682390161288No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTGGGAGCTGCCAACACTCT5470.10539275660824145No Hit
CTCCAGCCCCTTGCCCGGAGCCTGGCGGACCCAGTGCATGGCATAACTACTGAAGGTGAATCCAGACGCTGCACA5440.10481473417711763No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA5380.10365868931487002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)