FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1239000
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGGTACTACCGGCATATGAGC42980.34689265536723163No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGGTACTACCGGCATATGAGCAGCAGTAATAAT40970.3306698950766747No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38350.30952380952380953No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTCGTCTCCTGGT38300.30912025827280065No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37090.2993543179983858No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG32660.2635996771589992No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29940.24164648910411624No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA29860.241000807102502No Hit
GATTATTACTGCTGCTCATATGCCGGTAGTACCACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT27540.22227602905569008No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTCGTCTCCTGGTACCAACAGCACCCAGGCAAAGC26250.211864406779661No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25840.20855528652138824No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG24930.20121065375302666No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24770.1999192897497982No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23820.19225181598062954No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23660.19096045197740114No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGACTTATAACCTCGTCTCCTGGTACCAACAGC23000.18563357546408393No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT22610.1824858757062147No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGCATTGCCAGACTTGGAGCCAG21850.17635189669087972No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21830.17619047619047618No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC21770.17570621468926553No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21770.17570621468926553No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20930.16892655367231638No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20510.16553672316384183No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20210.16311541565778853No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC19530.1576271186440678No Hit
GGCTGGGGCCCAGAGATTGTCAGGGGGGCCGTGTTGCCAGACTTGGAGCC19180.15480225988700563No Hit
CTTATAACCTCGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAA18810.15181598062953994No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18770.15149313962873284No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGGTACTACCGGCATATGAGCAGCAGTAATAATC18600.15012106537530265No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCT18110.14616626311541567No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC17970.14503631961259078No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17920.14463276836158193No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC17730.14309927360774818No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA17420.14059725585149316No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG16890.13631961259079903No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGG16760.13527037933817593No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA16760.13527037933817593No Hit
CCCAAGTGGTACTACCGGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTC16540.13349475383373688No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG16270.13131557707828895No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16250.1311541565778854No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16030.12937853107344632No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15950.1287328490718321No Hit
GCCCCAGCCTGAGGACGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCA15580.12574656981436644No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15560.12558514931396286No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC15220.1228410008071025No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14950.12066182405165454No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC14900.12025827280064567No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14880.12009685230024213No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC14850.1198547215496368No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14750.11904761904761905No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGG14100.11380145278450363No Hit
TTGCCAGACTTGGAGCCAGAGAAGCGATCAGAAACGTCCTCAGGCTGGGG14060.11347861178369653No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACAC13830.1116222760290557No Hit
GCCTGAGGACGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAATGCGGCCTCCCTGACAATCTCTGGGCTCC13810.11146085552865215No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13690.11049233252623082No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC13660.1102502017756255No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACC13430.10839386602098466No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13420.10831315577078289No Hit
GTCTGGCAATGCGGCCTCCCTGACAATCTCTGGGCTCCAGGCTGAGGACG13230.10677966101694915No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGTCCTGAGTGAGG13180.10637610976594028No Hit
GTAATACATGGCGGTGTCCGAGGCCCTCAGGCTGCTCCACTGCAGGTAGGCGGTATTGATGGACTTGTCGGCTG13170.1062953995157385No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCA13110.10581113801452784No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT13110.10581113801452784No Hit
GTTGGTACCAGGAGACGAGGTTATAAGTCCCAACATCACTGCTGGTTCCAGTGCAGGAGATGGTGATCGACTGTC12810.10338983050847458No Hit
CCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC12510.10096852300242129No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACA12490.10080710250201774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)