FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998579
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37490.3754334909906978No Hit
GTATGATGACTGGCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT35400.3545037498285063No Hit
GTCATCATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT34780.34829492709139687No Hit
GTACGTAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACTCTGTATCTGGAAATGA33540.335877281617178No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT33530.3357771393149666No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC30990.3103409945532602No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGATACAGAGTGTTCTTGGAATTGTCTCTGG27130.2716860658996434No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23840.2387392484720788No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23140.23172928731727782No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22230.2226163378160366No Hit
GTCTACGTACTGTGTGGTATCACCAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT21870.21901121493642467No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTGGT21650.21680808428777293No Hit
CTCCTGCAGGGCCAGTCAGACTTTTGGTAGCCACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCT20540.2056922887423028No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG20130.20158645435163366No Hit
CATCTATGCTGCATCCACCAGGGCCTCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCGGGGACAGAGTTC19890.19918303909855906No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19630.19657933924106158No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCGGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTG18790.18816738585530038No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGATGACTGG18740.18766667434424317No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT18560.18586411290443722No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA18350.18376112455799692No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17490.17514888656781286No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17050.1707426252705094No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC16990.17014177145724074No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTGGTAGCCACTTAGCCTGGT16890.1691403484351263No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG16570.16593579476436016No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGCCAGTCATCATACTGCTGAC16370.1639329487201313No Hit
GTCTATTACTGTGCGAAACTTCTGGTAGTTGGTAGTAGTGGTTGGCGCTACTACTTTGACGACTGGGGCCAGGGA16330.16353237951128552No Hit
GCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGC16250.162731241093594No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15550.15572127993879303No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC14930.14951245720168357No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGCCAGTC14710.14730932655303186No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14400.14420491518447714No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14010.14029936539823087No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13950.13969851158496224No Hit
GTTTATTACTGTCAGCAGTATGATGACTGGCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA13010.13028513517708665No Hit
GCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGCTAAGTGGCTACCAAAAGTCTGACTGGCCCTGCAGGAG12940.12958413906160654No Hit
CTCCCAGGCTCCTCATCTATGCTGCATCCACCAGGGCCTCTGGTATCCCA12920.12938385445718364No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC12810.12828228913285777No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12550.1256785892753603No Hit
GAGTGAACTCTGTCCCCGACCCACTGCCACTGAACCTGGCTGGGATACCAGAGGCCCTGGTGGATGCAGCAT12520.12537816236872595No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12490.12507773546209164No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCCGACCCACTGCC12460.1247773085554573No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG12310.12327517402228567No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12300.12317503172007423No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC12150.12167289718690258No Hit
GGATACCAGAGGCCCTGGTGGATGCAGCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGG11840.11856848581834788No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT11780.11796763200507922No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAAT11740.11756706279623344No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC11570.11586464365863891No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11520.11536393214758173No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11510.11526378984537027No Hit
GTCGGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11370.11386179761441008No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTTATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCC11040.11055710164143248No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10980.10995624782816381No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT10970.10985610552595237No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG10960.10975596322374094No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10930.1094555363171066No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10900.10915510941047228No Hit
GGTTTCTGCTGGTACCAGGCTAAGTGGCTACCAAAAGTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC10880.10895482480604939No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGACTGCGTCATAACTATTTCTCC10670.1068518364596091No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10570.10585041343749467No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10550.10565012883307179No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10400.10414799429990017No Hit
GTTCAGTGGCAGTGGGTCGGGGACAGAGTTCACTCTCACCATCAGCAGCC10160.10174457904682553No Hit
GCAGAAACCTGGGCAGGCTCCCAGGCTCCTCATCTATGCTGCATCCACCAGGGCCTCTGGTATCCCAGCCAGGTT10070.10084329832692257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)