FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998579
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36350.36401726853859334No Hit
GTATGATGACTGGCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT32250.3229589246319019No Hit
GTACGTAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACTCTGTATCTGGAAATGA31070.3111421329709517No Hit
GTCATCATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT28890.28931111108885726No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC28660.2870078381379941No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT27840.2787961693566558No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGATACAGAGTGTTCTTGGAATTGTCTCTGG24530.24564906732466837No Hit
CTCCTGCAGGGCCAGTCAGACTTTTGGTAGCCACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCT22500.22532017997574555No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22120.2215147724917107No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20930.20959783852854907No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTGGT20490.2051915772312456No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA20190.20218730816490235No Hit
GTCTACGTACTGTGTGGTATCACCAATACTTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGT19780.19808147377423316No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19660.19687976614769587No Hit
CATCTATGCTGCATCCACCAGGGCCTCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCGGGGACAGAGTTC19320.19347492787250684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG18870.1889685242729919No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATGATGACTGG18470.18496283218453421No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCGGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTG17350.17374689433685267No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT16960.1698413445506064No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC16670.16693721778647458No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTTTTGGTAGCCACTTAGCCTGGT16550.16573551015993726No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16310.16333209490686265No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC15690.15712327216975322No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15630.15652241835648456No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG15600.15622199144985024No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGCCAGTCATCATACTGCTGAC15210.15231644166360397No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC15080.1510145917348552No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14450.14470562669553436No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14420.1444051997889No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG14010.14029936539823087No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC13900.13919780007390503No Hit
GTCTATTACTGTGCGAAACTTCTGGTAGTTGGTAGTAGTGGTTGGCGCTACTACTTTGACGACTGGGGCCAGGGA13810.13829651935400203No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTTATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCC13580.13599324640313884No Hit
GCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGC13310.13328940424342992No Hit
CTCCCAGGCTCCTCATCTATGCTGCATCCACCAGGGCCTCTGGTATCCCA13190.1320876966168926No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGTGGCCAGTC13100.13118641589698962No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12810.12828228913285777No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12670.12688029690189762No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12630.12647972769305182No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC12340.12357560092892No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT12340.12357560092892No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA12330.12347545862670854No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAAT12300.12317503172007423No Hit
GTTTATTACTGTCAGCAGTATGATGACTGGCCACTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA12220.12237389330238269No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC12170.12187318179132547No Hit
GCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGCTAAGTGGCTACCAAAAGTCTGACTGGCCCTGCAGGAG11740.11756706279623344No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCCGACCCACTGCC11560.11576450135642748No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATGACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG11490.11506350524094738No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11360.11376165531219863No Hit
GTCGGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT11220.11235966308123845No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACACAGACAGGGTGGCTGGAGACTGCGTCATAACTATTTCTCC11180.11195909387239267No Hit
ATGCTGCATCCACCAGGGCCTCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCGGGGACAGAGTTCACTCTC10920.10935539401489516No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT10890.10905496710826085No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10870.10885468250383795No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC10830.10845411329499217No Hit
GAGTGAACTCTGTCCCCGACCCACTGCCACTGAACCTGGCTGGGATACCAGAGGCCCTGGTGGATGCAGCAT10730.10745269027287775No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC10690.10705212106403199No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10640.10655140955297478No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10450.10464870581095737No Hit
GGATACCAGAGGCCCTGGTGGATGCAGCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGG10340.1035471404866315No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10140.10154429444240265No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10070.10084329832692257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)