FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661762
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCATCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCT19920.3010145641484401No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG18640.2816722628376969No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGGCTGTCATCCCATGATGCACAGTAAT17970.2715477769953548No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAG17800.26897887760252176No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGAAGATAGAGCGTGTTCTTGGAATTGTCTCTGG17460.2638410788168556No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16700.25235658741360184No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16110.2434409954031812No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG15930.24072098428135794No Hit
GTCCTGGGCCCAGTCTGTGCTGGCTCAGCCACCCTCTGCGTCTGGGACCC15020.2269698169432515No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA13650.20626751007159672No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13350.20173415820189133No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG12100.18284519207811872No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12000.1813340747882169No Hit
GTATATTACTGTGCGAGAGGCCCGATGACTACAGTTATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC11290.1706051420299141No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC11270.17030291857193372No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT11170.16879180128203192No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11110.16788513090809082No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGGCTCAGCCACCCTCTGCGTCT11100.16773401917910066No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10900.16471178459929703No Hit
GTGCTGGCTCAGCCACCCTCTGCGTCTGGGACCCCCGGGCAGAGGGTCGCCATCTCTTGTTCTGGAAGCAGCTCC10670.16123621483252287No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG10430.15760953333675853No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10400.15715619814978798No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCTTGGGAGCTGCTGGTACCAGTTT10300.15564508085988618No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGGCTCAGCCACCCTCTGCGTCTGGGACCCCCGGGC10210.15428507529897456No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGGCTGTCATCCCATGATGCACAGTAATA10060.15201839936412184No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10050.15186728763513166No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9660.1459739302045146No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC9630.14552059501754408No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC9540.14416058945663246No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9380.14174280179278956No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTCTATCTT8950.13524499744621177No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCC8670.13101386903448672No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTCAGGGACC8660.13086275730549654No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC8600.12995608693155547No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8410.12708496408074202No Hit
GTATTACTTCCGACGTTGGAGCTGCTTCCAGAACAAGAGATGGCGACCCT8330.12587607024882055No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8260.12481828814588931No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAATGGTCCGGTGTTCGGCGGAGGGAC8220.1242138412299286No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8050.12164494183709551No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA8010.12104049492113479No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCTTGGGAGCTGCTGGTACCAGTTTACAGTATTACTTCCG7990.12073827146315444No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC7950.1201338245471937No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTC7940.11998271281820352No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCC7850.1186227072572919No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT7840.11847159552830172No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7730.11680936650940972No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC7640.11544936094849811No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG7610.11499602576152756No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA7590.11469380230354718No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC7470.11288046155566503No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7440.1124271263686945No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGAC7210.10895155660192032No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC7200.10880044487293014No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG7150.10804488622797924No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7120.1075915510410087No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7110.10744043931201852No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7080.10698710412504799No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTG7080.10698710412504799No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT6980.10547598683514617No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6930.10472042819019527No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6900.10426709300322473No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6880.10396486954524436No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6860.10366264608726401No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC6860.10366264608726401No Hit
GTCTGTGCTGGCTCAGCCACCCTCTGCGTCTGGGACCCCCGGGCAGAGGGTCGCCATCTCTTGTTCTGGAAGC6840.10336042262928365No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA6780.10245375225534255No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC6680.10094263496544076No Hit
CCCAAGAACGGCCCCCAAACTCCTCATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG6630.10018707632048983No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6620.10003596459149966No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)