FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877049
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGATGACCACTGGAGTTGGGTCCGCCAGCC31580.36007110207069387No Hit
GCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23820.27159257920595087No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA21050.24000939514211864No Hit
GCCCTGGGCTGACCTAGGACGGACAGCTTGGTCCCTCTGCCGAACACCCAAGCATGTAGGCTGCTATCCCATGTT19820.22598509319319673No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19730.22495892475790977No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA16420.18721873008235573No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC14680.16737947366680767No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCCTGGGCTGACCTAGGACGGACAGCTTGGTCCCT14600.1664673239465526No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14450.1647570432210743No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13530.15426732143814084No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT13360.1523290032825988No Hit
TCCTAGGTCAGCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12770.14560189909571758No Hit
CCCAGAAACAGCCCCCAAACTCCTCATTTATGACAACAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG12730.14514582423559003No Hit
GTCCTAGGTCAGCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12550.1430934873650161No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC12540.1429794686499842No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG12470.142181337644761No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC12350.14081311306437838No Hit
GGACAGAAGGTCACCATCTCGTGCTCTGGAAGCAGCTCCAATATTGAGAGTAATTATGTATCCTGGTACCAGTAC12080.13773460775851748No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11330.12918320413112608No Hit
GACCTAGGACGGACAGCTTGGTCCCTCTGCCGAACACCCAAGCATGTAGGCTGCTATCCCATGTTCCGCAGTGAT11170.12735890469061592No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11160.12724488597558403No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCGTGCTCTGG11080.12633273625532895No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCCTGGG10990.125306567820042No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA10860.12382432452462747No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG10770.1227981560893405No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG10750.12257011865927674No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10720.12222806251418109No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG10580.12063180050373469No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC10520.11994768821354337No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC10040.11447478989201286No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC9970.11367665888678967No Hit
GATTATCACTGCGGAACATGGGATAGCAGCCTACATGCTTGGGTGTTCGGCAGAGGGACCAAGCTGTCCGTCCT9970.11367665888678967No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9930.11322058402666213No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTGTGGCGGT9910.11299254659659837No Hit
GTACCAGTACCTCCCAGAAACAGCCCCCAAACTCCTCATTTATGACAACAATAAGCGACCCTCAGGGATTCCTG9910.11299254659659837No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9880.1126504904515027No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9870.11253647173647083No Hit
GTGATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCCAG9710.11071217229596067No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA9660.11014207872080124No Hit
GTACTGGTACCAGGATACATAATTACTCTCAATATTGGAGCTGCTTCCAGAGCACGAGATGGTGACCTTCTGTC9660.11014207872080124No Hit
GTTTCTGGGAGGTACTGGTACCAGGATACATAATTACTCTCAATATTGGAGCTGCTTCCAGAGCACGAGATGGTG9410.1072916108450041No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGCGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC9260.10558133011952582No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAGGGCTTCAACAGTCCTGCGCCCCACTGCTGTAGCTGCAC9210.10501123654436641No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9130.1040990868241113No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC9070.10341497453391998No Hit
ACTCCAGACTGGGGACGAGGCCGATTATCACTGCGGAACATGGGATAGCAGCCTACATGCTTGGGTGTTCGGC9070.10341497453391998No Hit
GTGTATTACTGTGCGAGACAGAGACGTGGCAGTGGCTGGTACTTTCCTTGGTACTTCGATCTCTGGGACCGTGGC9000.1026168435286968No Hit
ACATGGGATAGCAGCCTACATGCTTGGGTGTTCGGCAGAGGGACCAAGCTGTCCGTCCTAGGTCAGCCCAGGG8980.10238880609863303No Hit
CAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCAAGTCTGGCACGTCAGCCACCCTGGGCATCAC8860.10102058151825041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)