FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877049
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGATGACCACTGGAGTTGGGTCCGCCAGCC28120.3206206266696615No Hit
GCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23660.26976827976544066No Hit
GCCCTGGGCTGACCTAGGACGGACAGCTTGGTCCCTCTGCCGAACACCCAAGCATGTAGGCTGCTATCCCATGTT21620.24650846189893608No Hit
GTAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA19130.21811780185599663No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCGTG18360.20933836079854148No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17920.2043215373371385No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA17100.19497200270452392No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCGTGCTCTGGAAGCAGCTCC15140.1726243345582744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13230.1508467599871843No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT13200.15050470384208864No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC13180.15027666641202486No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCCTGGGCTGACCTAGGACGGACAGCTTGGTCCCT12980.14799629211138715No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12780.14571591781074947No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12540.1429794686499842No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC11490.13100750357163626No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11470.1307794661415725No Hit
GACCTAGGACGGACAGCTTGGTCCCTCTGCCGAACACCCAAGCATGTAGGCTGCTATCCCATGTTCCGCAGTGAT11370.1296392789912536No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG11320.1290691854160942No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC11030.12576264268016954No Hit
TCCTAGGTCAGCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10710.1221140437991492No Hit
GTCCTAGGTCAGCCCAGGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10670.12165796893902166No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC10480.11949161335341582No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG10250.11686918290768247No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9940.11333460274169402No Hit
GGACAGAAGGTCACCATCTCGTGCTCTGGAAGCAGCTCCAATATTGAGAGTAATTATGTATCCTGGTACCAGTAC9860.11242245302143894No Hit
GTATCAACGCAGAGTACGGGAAGCTCTGCTTCAGCTGTGAGCGCAGAAGG9820.1119663781613114No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9810.11185235944627951No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9800.11173834073124762No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC9720.11082619101099254No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCCTGGG9470.1079757231351954No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC9440.10763366699009976No Hit
CCCAGAAACAGCCCCCAAACTCCTCATTTATGACAACAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG9270.10569534883455771No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCGTGCTCTGG9250.10546731140449395No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGCGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC9250.10546731140449395No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9210.10501123654436641No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA8980.10238880609863303No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8950.10204674995353738No Hit
GATTATCACTGCGGAACATGGGATAGCAGCCTACATGCTTGGGTGTTCGGCAGAGGGACCAAGCTGTCCGTCCT8800.1003364692280591No Hit
ACTCCAGACTGGGGACGAGGCCGATTATCACTGCGGAACATGGGATAGCAGCCTACATGCTTGGGTGTTCGGC8790.10022245051302722No Hit
ATCTTCATCATGACCTGCTCCCCTCTCCTCCTCACCCTTCTCATTCACTG8780.10010843179799533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)