FastQCFastQC Report
Wed 8 May 2024
EGAF00004717829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964941
Total Bases70 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGGTACA31770.32924292780594877No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29900.3098635046080538No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27610.2861314836865674No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG25520.2644721283477436No Hit
CCCTTGGACAACCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTA22130.22934044672161302No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC22000.22799321409288234No Hit
GTACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACGACG21520.2230188166944922No Hit
GTCTATTACTGTGTGAGAGAATACTGCAGTGGTGGCACGTGCTACCAACC21360.22136068422836216No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20080.20809562449932173No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG20050.20778472466192233No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19630.20343212693833096No Hit
GTGATAGAAACACCTACTTGCACTGGTTTCAGCACAGGCCAGGCCAATCT19400.20104856151826897No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19050.1974213967486095No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18220.18881983458055987No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17640.18280910439083842No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17530.18166913832037399No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17410.18042553897077646No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16870.17482934189758753No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16350.16944041138266486No Hit
ACATAGTACTGTTCACTTCCACGTTGCTTTATGTTGGCCACCCACTCCAG16210.16798954547480105No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTG15760.16332604791381028No Hit
GTACACGCTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA15740.163118781355544No Hit
GGCCTGGCCTGTGCTGAAACCAGTGCAAGTAGGTGTTTCTATCACTGTGT15710.16280788151814463No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15630.1619788152850796No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15620.1618751820059465No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15120.15669351804929005No Hit
GTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCACTGATTTCACACTGAA14930.15472448574576061No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT14860.1539990527918287No Hit
CAGTAATATATTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGT14810.15348088639616309No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAGTCAC14740.15275545344223118No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14640.1517191206508999No Hit
ATATTACTGCATGCAAGGTACACGCTGGCCCTGGACGTTCGGCCAAGGGA14530.1505791545804355No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGATTTGGGGCTGAGCTGGG14370.14892102211430544No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA14260.14778105604384104No Hit
AGCGTGTACCTTGCATGCAGTAATATATTCCAACATCCTCAGCCTCCACC14020.14529385734464592No Hit
GGTTTCAGCACAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTT13670.14166669257498646No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13420.13907586059665825No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13070.13544869582699876No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC12880.1334796635234693No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT12820.13285786384867054No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12770.1323396974530049No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC12660.13119973138254049No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12640.13099246482427424No Hit
GGACTGGAGTGGGTGGCCAACATAAAGCAACGTGGAAGTGAACAGTACTA12620.13078519826600796No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12310.127572566612881No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTAGTGACTATTGGATGAGTTGGG12240.12684713365894912No Hit
TTCTATCACTGTGTACGAGGCTTTGACTAGACTTGCAGGAGATGGAGGCC12170.1261217007050172No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC12030.1246708347971534No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT11830.12259816921449083No Hit
GTGTACGAGGCTTTGACTAGACTTGCAGGAGATGGAGGCCGGTTGTCCAA11750.12176910298142582No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGTTGACTCAGT11440.11855647132829883No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC11410.11824557149089944No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11370.11783103837436694No Hit
CTCGTACACAGTGATAGAAACACCTACTTGCACTGGTTTCAGCACAGGCC11300.11710560542043504No Hit
ACATAAAGCAACGTGGAAGTGAACAGTACTATGTGGACTCTGTGAAGGGC11180.11586200607083749No Hit
GCTTGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGGTTG11160.11565473951257123No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC11150.1155511062334381No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT11130.11534383967517184No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAG11010.11410024032557431No Hit
GGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG11010.11410024032557431No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10650.11036944227678169No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10620.1100585424393823No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC10540.10922947620631727No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCAACACAACATCC10510.10891857636891789No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10470.10850404325238537No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10460.10840040997325226No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAACCGGCCTCCATCTCCT10350.10726044390278783No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTG10330.10705317734452159No Hit
GTGCTGAAACCAGTGCAAGTAGGTGTTTCTATCACTGTGTACGAGGCTTT10300.1067422775071222No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGGTA10300.1067422775071222No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCA10240.10612047783232342No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTG10110.10477324520359275No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC10040.10404781224966086No Hit
GTAATATATTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGA9840.10197514666699829No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGCCAGGTTGGT9830.10187151338786515No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT9780.10135334699219953No Hit
GCACTGAGCACAGAGGACTCACCATGGATTTGGGGCTGAGCTGGGTTTTC9690.10042064748000136No Hit
CTCCAGAGACGACGCCAAGAACTCAGTGTACCTGCAAATGAACAGCCTGA9660.10010974764260198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)