FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences964941
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGGTACACTGAGTTCTTGGCGTCGTCTCTGG26440.2740063900279914No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25560.26488666146427603No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23930.24799443696557613No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22590.23410757756173695No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT20170.20902832401151986No Hit
GTACACGCTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA18640.19317243230415124No Hit
GTCTATTACTGTGTGAGAGAATACTGCAGTGGTGGCACGTGCTACCAACC18370.19037433376755677No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC17520.18156550504124086No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16490.17089127729052864No Hit
GTACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACGACG16470.1706840107322624No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16030.16612414645040474No Hit
ATATTACTGCATGCAAGGTACACGCTGGCCCTGGACGTTCGGCCAAGGGA15690.16260061495987838No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA15290.15845528379455323No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC15120.15669351804929005No Hit
GTGATAGAAACACCTACTTGCACTGGTTTCAGCACAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT14900.15441358590836124No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14470.14995735490563672No Hit
CCCTTGGACAACCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTACACAGTGATAGAAACACCTACTTGC14360.14881738883517232No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13590.14083762634192143No Hit
CAGTAATATATTCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCCG13420.13907586059665825No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13420.13907586059665825No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT13290.13772862796792756No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGG12900.13368693008173557No Hit
AGCGTGTACCTTGCATGCAGTAATATATTCCAACATCCTCAGCCTCCACC12880.1334796635234693No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAACTCATCCAATAGTCACTAAACGTGAATCCAGAGGCTGCAC12730.1319251643364724No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG12520.12974886547467668No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC12470.12923069907901105No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTGTGCTGAA12410.12860889940421227No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG12340.1278834664502804No Hit
ACATAGTACTGTTCACTTCCACGTTGCTTTATGTTGGCCACCCACTCCAGTCCCTTCCCTGGAGCCTGGCGGACC12050.12487810135541966No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11900.12332360216842272No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGATTTGGGGCTGAGCTGGG11820.12249453593535771No Hit
GGCCTGGCCTGTGCTGAAACCAGTGCAAGTAGGTGTTTCTATCACTGTGTACGAGGCTTTGACTAGACTTGCAGG11790.12218363609795832No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC11530.11948917084049698No Hit
GTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCACTGATTTCACACTGAA11440.11855647132829883No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11430.1184528380491657No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAACCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTAC11410.11824557149089944No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC11380.11793467165350005No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11380.11793467165350005No Hit
GGTTTCAGCACAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCC11260.11669107230390251No Hit
CTCCTGTGCAGCCTCTGGATTCACGTTTAGTGACTATTGGATGAGTTGGGTCCGCCAGGCTCCAGGGAAGGG11130.11534383967517184No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10930.11327117409250928No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAGGGCCAGCGTGTACCTTGCATGC10860.11254574113857738No Hit
CTCCCTGCCCGTCACCCTTGGACAACCGGCCTCCATCTCCTGCAAGTCTAGTCAAAGCCTCGTACACAGTGAT10580.1096440093228498No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC10460.10840040997325226No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10450.10829677669411913No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10150.10518777832012527No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9990.10352964585399521No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCC9960.10321874601659582No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT9870.10228604650439768No Hit
GGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC9860.10218241322526454No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9680.10031701420086825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)