FastQCFastQC Report
Wed 8 May 2024
EGAF00004717847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007591
Total Bases74.4 Mbp
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA43340.4301348463811209No Hit
GTCTATTACTGTGTGAGAGGGATGTATTGTGGTGGTGATTGCTACCCGCC30360.3013127350283994No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26640.2643929927917181No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24740.24553613519771417No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24230.24047455763300785No Hit
ATACTACACAGACTCAGTGAAGGGCCGGTTCACCATCTCCAGAGACAACG23500.2332295544521537No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG22120.2195335210417719No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21710.2154644096662237No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA21580.2141742036203182No Hit
GTGTAGTATATGTAACTGCTTTTACTGCTGGTGGATGAGACCCACTCCAG19410.1926376873155874No Hit
GCCTTGGGCTGACCGAGGACGGTGACCTTGGTCCCACTTCCCATGACATA19000.18856857594003915No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18340.1820182990915957No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA18250.1811250795213534No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18210.18072809304569018No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18080.17943788699978463No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC17940.17804843433496328No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17030.16901699201362458No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC16970.1684215123001297No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT16710.16584110020831866No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16660.1653448671137396No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16470.1634591813543392No Hit
GTCTTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC16380.1625659617840969No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATACCATGAACTGGG16360.1623674685462653No Hit
GATTATTATTGTGCTTCATGGGATGACAGCCTAAACTATTATGTCATGGG15900.15780212407613806No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG15880.15760363083830642No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15860.15740513760047478No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC15710.15591643831673765No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA15560.1544277390330005No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15430.15313753298709495No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15210.15095410737094714No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15180.1506563675141997No Hit
GTGCAGGGTCTTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT14670.1455947899494934No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC14560.1445030771414195No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14480.143709104190093No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC14380.1427166380009349No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG14210.14102944547936613No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA14130.14023547252803964No Hit
CCACAATACATCCCTCTCACACAGTAATAGACAGCCGTGTCCTCGGCTCT14030.13924300633888156No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13820.13715882734164953No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13740.13636485439032306No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCCATTTTAGAAGGT13550.13447916863092266No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13480.133784442298512No Hit
CCTCACTCACTGTGCAGGGTCTTGGGCCCAGTCTGTGCTGACTCAGCCAC13360.13259348287152228No Hit
ATAATAATCAGCCCCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG13300.1319980031580274No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC13220.13120403020670093No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG13210.13110478358778513No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG13060.12961608430404797No Hit
CCCCTGAGGGCCGCTGATTATTCCTAAAGATGAGGAGTTTGGGGGCCGTT12980.1288221113527215No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGA12650.12554697292849976No Hit
GTAGTTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAG12530.12435601350151003No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG12490.1239590270258468No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12460.12366128716909937No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12440.12346279393126774No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGG12370.12276806759885708No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT12280.12187484802861479No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12240.12147786155295155No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12180.12088238183945668No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGTATAACTAC12040.11949292917463533No Hit
CTATTATGTCATGGGAAGTGGGACCAAGGTCACCGTCCTCGGTCAGCCCA12000.1190959426989721No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC11950.11859970960439305No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG11940.11850046298547724No Hit
ACATAATAGTTTAGGCTGTCATCCCATGAAGCACAATAATAATCAGCCCC11850.11760724341523494No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG11710.11621779075041362No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11570.11482833808559227No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11460.11373662527751835No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC11430.11343888542077092No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11390.11304189894510769No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATACC11160.11075922671004407No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGTGACCTTGGTC11150.11065998009112823No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11090.11006450037763338No Hit
GGGCTGGAGTGGGTCTCATCCACCAGCAGTAAAAGCAGTTACATATACTA11070.10986600713980177No Hit
CTATTACTGTGTGAGAGGGATGTATTGTGGTGGTGATTGCTACCCGCCTG11000.10917128080739108No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA10920.10837730785606461No Hit
CAATACATCCCTCTCACACAGTAATAGACAGCCGTGTCCTCGGCTCTCAG10890.10807956799931719No Hit
GTATTGTGGTGGTGATTGCTACCCGCCTGGGGGCATGGACGTCTGGGGCC10860.10778182814256976No Hit
GTATAACTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA10690.10609463562100098No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT10640.10559840252642194No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG10610.1053006626696745No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC10590.10510216943184288No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT10490.10410970324268479No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10490.10410970324268479No Hit
GTGTGAGGTGCAGTTGGTAGAGTCTGGGGGAGGCCTGGTCAAGCCTGGGG10320.102422510721116No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATT10300.1022240174832844No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTTTAGGAATAATCAGCGGCCCT10220.1014300445319579No Hit
GAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCCATTTTAGAAGGTGTCCA10170.10093381143737884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)