FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007591
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGACTTCTTGTCGTTGTCTCTGGA31290.31054267058756974No Hit
GTCTATTACTGTGTGAGAGGGATGTATTGTGGTGGTGATTGCTACCCGCCTGGGGGCATGGACGTCTGGGGCCAA27360.2715387493536564No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT21840.21675461571212923No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT20620.2046465282044004No Hit
ATACTACACAGACTCAGTGAAGGGCCGGTTCACCATCTCCAGAGACAACGACAAGAAGTCACTGTATCTGCAAAT20200.20047817020993636No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG18850.18707987665630202No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18340.1820182990915957No Hit
GTCTTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC17160.17030719805953012No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC16170.1604817827868649No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15900.15780212407613806No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15860.15740513760047478No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15650.1553209586032428No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15480.153633766081674No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC14900.14787746218455702No Hit
GTGTAGTATATGTAACTGCTTTTACTGCTGGTGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC14900.14787746218455702No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG14860.14748047570889378No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGG14840.14728198247106217No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14650.14539629671166177No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG14090.13983848605237642No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13890.13785355367406021No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC13820.13715882734164953No Hit
GCCTTGGGCTGACCGAGGACGGTGACCTTGGTCCCACTTCCCATGACATAATAGTTTAGGCTGTCATCCCATGAA13560.1345784152498385No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGTGACCTTGGTCCCA13510.13408218215525944No Hit
GTGCAGGGTCTTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT13400.1329904693471855No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13260.13160101668236418No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC13100.13001307077971122No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC12740.12644019249874205No Hit
CCTCACTCACTGTGCAGGGTCTTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA12680.1258447127852472No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG12640.12544772630958395No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGACTTCTTGTCGTTGTCTCTG12450.12356204055018356No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCCATTTTAGAAGGTGTCCAGTGTGAGGTGCAGTTGGTA12290.12197409464753059No Hit
GATTATTATTGTGCTTCATGGGATGACAGCCTAAACTATTATGTCATGGGAAGTGGGACCAAGGTCACCGTCCTC12240.12147786155295155No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG12070.11979066903138277No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA11810.11721025693957171No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11590.1150268313234239No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11570.11482833808559227No Hit
CCACAATACATCCCTCTCACACAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGT11500.11413361175318161No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT11420.11333963880185512No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC11320.11234717261269703No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGG11070.10986600713980177No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11010.10927052742630691No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT10970.10887354095064365No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10970.10887354095064365No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGGTATAACTACTGAAGGTGAATCCAGAGGCTGCACA10960.10877429433172785No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10950.10867504771281204No Hit
CTATTATGTCATGGGAAGTGGGACCAAGGTCACCGTCCTCGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTT10950.10867504771281204No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG10850.10768258152365395No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10820.10738484166690652No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA10820.10738484166690652No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG10690.10609463562100098No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT10660.10579689576425354No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGC10590.10510216943184288No Hit
GTAGTTATACCATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCTCATCCACCAGCAGTAAAA10430.10351422352918992No Hit
CCCCTGAGGGCCGCTGATTATTCCTAAAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGTATA10390.10311723705352668No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT10140.10063607158063144No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)