FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359694
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41460.3049215485248887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT37110.27292905609644524No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC34290.2521890954876612No Hit
GCGTTACACCTGGCCGGCCGGAACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT31420.23108140508084907No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATT28580.2101943525528538No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCA28460.2093118010375864No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27630.20320748639032016No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTGTT27440.20181011315781347No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTCGCTCTTTTAGAAGGTCTCCAGTGTGAGGTGCAGCTGGTGGAGT27260.20048628588491235No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26940.1981328151775326No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26660.1960735283085753No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG24510.18026114699336765No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23630.1737891025480733No Hit
GTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC23630.1737891025480733No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23150.17025889648700368No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22610.16628741466830035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21630.15907991062694987No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21170.1556967964850915No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG21140.15547615860627464No Hit
GTTTATTACTGTCTGCAGCGTTACACCTGGCCGGCCGGAACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGA20870.15349041769692298No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT20500.15076921719151515No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA20390.14996021163585335No Hit
GGATAGGGTGGAACTCCCACCCACTCTCTCACACAATAATAAAGAGCCGT20240.1488570222417691No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19940.1466506434536006No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19530.1436352591097703No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19490.14334107527134782No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTTCCGGCCGGCCAGGTGTAACGCTGC19390.14260561567529165No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC19340.14223788587726358No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC19270.14172306416002423No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG19250.141575972240813No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19010.1398108692102782No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTCCTTAGCCTGGT18940.13929604749303887No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCGGTTACGATCTAAACTGGGTCCGCCAGGCTCCAGGGAAGGGG18390.13525101971472991No Hit
AGCCAGGATAGGGTGGAACTCCCACCCACTCTCTCACACAATAATAAAGA18000.13238272729011086No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17150.1261313207236334No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17060.12546940708718285No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17050.12539586112757722No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG16960.12473394749112669No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16920.12443976365270422No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16780.12341012021822557No Hit
CTCCAGAGACAACGCCAAGAACTCACTATATCTGCAAATGAACAGCCTGA16770.12333657425861995No Hit
CCACTGAACACAGAGGACTCAGCATGGAGTTGGGGCTGTGCTGGGTTTTCCTTGTCGCTCTTTTAGAAGGTCTCC16700.12282175254138064No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGG16560.121792109106902No Hit
CAATAATAAAGAGCCGTGTCTTCGACTGTCAGGCTGTTCATTTGCAGATA16450.1209831035512402No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16380.12046828183400089No Hit
ATCCTGGCTGGTTCGACCCCTGGGGCCAGGGGACCCTGGTCACCGTCTCC16100.11840899496504362No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16100.11840899496504362No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16080.11826190304583238No Hit
GTGTAACGCTGCAGACAGTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTC16060.11811481112662114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTTCCGGCCGGCC15970.11745289749017059No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15880.11679098385372004No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC15500.11399623738870658No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15360.11296659395422795No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCC15230.1120104964793549No Hit
GTGCTGGGTTTTCCTTGTCGCTCTTTTAGAAGGTCTCCAGTGTGAGGTGC15180.11164276668132683No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14900.10958347981236956No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATCATGCATCTAACAGGGCCACTGGCATCCCAGCCAGATT14740.10840674445867968No Hit
GTACTACGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACG14680.10796546870104598No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT14610.10745064698380664No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14610.10745064698380664No Hit
ATAATAAAGAGCCGTGTCTTCGACTGTCAGGCTGTTCATTTGCAGATATAGTGAGTTCTTGGCGTTGTCTCTGG14420.10605327375129993No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC14390.10583263587248307No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC14360.10561199799366623No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTGTTAGATGCATGAT14310.10524426819563813No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14240.10472944647839882No Hit
GACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATTTTGCAGTTTATTACTGTCTGCAGCGTTACACCTGG14200.10443526263997635No Hit
CCTTGGGGCTGGTCGGGGATGTTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGGGGTCGAACCAGCCAGGAT14160.10414107880155389No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC14020.10311143536707525No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13820.1016405161749629No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGTTGAGGAGACGGTGACCAGGGTCCCCTGG13760.10119924041732921No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13750.10112569445772357No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13670.10053732678087864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)