FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359694
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42030.3091136682224089No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT31960.23505288689955242No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC29550.21732831063459865No Hit
GCGTTACACCTGGCCGGCCGGAACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT29500.21696058083657058No Hit
GTGTAACGCTGCAGACAGTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTC29050.21365101265431782No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTCGAGCCTGAAGATT27140.19960373436964493No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTCGCTCTTTTAGAAGGTCTCCAGTGTGAGGTGCAGCTGGTGGAGT25520.18768928891353495No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25430.1870273752770844No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTGTT24830.18261461770074738No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCA24490.1801140550741564No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24250.17834895204362156No Hit
GTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC23090.16981762072936998No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22990.1690821611333138No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA22470.1652577712338217No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG22310.16408103588013184No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTTCCGGCCGGCCAGGTGTAACGCTGC20590.1514311308279657No Hit
GTTTATTACTGTCTGCAGCGTTACACCTGGCCGGCCGGAACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGA20310.1493718439590084No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20270.14907766012058596No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20180.1484157464841354No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19870.14613582173636125No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19760.14532681618069948No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT19580.1440029889077984No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT19150.14084051264475683No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18970.13951668537185571No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18780.138119312139349No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCGGTTACGATCTAAACTGGGTCCGCCAGGCTCCAGGGAAGGGG18530.13628066314920856No Hit
GGATAGGGTGGAACTCCCACCCACTCTCTCACACAATAATAAAGAGCCGT18480.1359129333511805No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18360.1350303818359131No Hit
GTCTTTGTCTCCAGGGGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTCCTTAGCCTGGT18290.13451556011867374No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC18200.1338536464822232No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG18110.13319173284577265No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18050.13275045708813896No Hit
CTCCAGAGACAACGCCAAGAACTCACTATATCTGCAAATGAACAGCCTGA17850.1312795378960266No Hit
GTGCTGGGTTTTCCTTGTCGCTCTTTTAGAAGGTCTCCAGTGTGAGGTGC17490.12863188335022438No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG17110.12583713688521092No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16760.12326302829901434No Hit
GGTGTAACGCTGCAGACAGTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGT16730.12304239042019748No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTTCCGGCCGGCC16630.1223069308241413No Hit
AGCCAGGATAGGGTGGAACTCCCACCCACTCTCTCACACAATAATAAAGA16310.11995346011676156No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCC16290.11980636819755033No Hit
ATCCTGGCTGGTTCGACCCCTGGGGCCAGGGGACCCTGGTCACCGTCTCC16020.11782062728819867No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15880.11679098385372004No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15880.11679098385372004No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG15590.11465815102515713No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15440.11355496163107288No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15430.11348141567146726No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCGAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC15310.11259886415619985No Hit
CCACTGAACACAGAGGACTCAGCATGGAGTTGGGGCTGTGCTGGGTTTTCCTTGTCGCTCTTTTAGAAGGTCTCC14870.1093628419335527No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14780.10870092829710215No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14660.10781837678183474No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTGTTAGATGCATGAT14540.10693582526656734No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT14500.10664164142814488No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14430.10612681971090554No Hit
CAATAATAAAGAGCCGTGTCTTCGACTGTCAGGCTGTTCATTTGCAGATA14020.10311143536707525No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13900.10222888385180784No Hit
GGATAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAACTCCTTAGCCTGGTACCAACAGAAGCCTGG13860.10193470001338538No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC13820.1016405161749629No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC13800.10149342425575167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)