FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933010
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT30890.33107898093268023No Hit
GTAATACACAGCCGTGTCCTCGACTTTCAGGCTGTTCATTTGCAGATACA25740.2758812874460081No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23980.25701760967192205No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG22810.24447755115164896No Hit
GTACACAGTGGCCTTGGACGTTCGGCCAAGGGACCAGCGTGGAAATCGAACGAACTGTGGCTGCACCATCTGTCT21430.22968671289696788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT20410.21875435418698622No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19630.21039431517347082No Hit
CCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA19360.20750045551494625No Hit
GTGTATTACTGTGCGAGAGGTCGTATAATTGGAATGACGGGAGGGGGAACCCTGGTCACCGTCTCCTCAGGGAG17030.18252751846175283No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC16730.17931211884117No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC16480.17663261915735093No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16330.17502491934705952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15880.17020181991618524No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15830.16966591997942146No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15800.16934438001736316No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAG15440.16548590047266376No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGA15220.163127940750903No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14770.15830484132002873No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14660.15712586145914834No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14620.1566971415097373No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13940.14940890236974952No Hit
ACTGTGTACCTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAG13880.14876582244563297No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13300.1425493831791728No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAAAAATTAG13290.14244220319182002No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12610.13515396405183222No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGTGATGTTGTGGTGACTCAGTCTCCACTCTCCCTGCCCGTCACCCT12550.13451088412771567No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12480.13376062421624632No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG12220.13097394454507455No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC12000.1286159848233138No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGTGATGTTGTGGTGACTCAGTCTCCACTCTCC11970.12829444486125552No Hit
GTGTACCTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGC11830.12679392503831685No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT11780.12625802510155304No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACGCTGGTCCCTTGGCCGAACGTC11750.12593648513949476No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC11720.12561494517743646No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11710.1255077651900837No Hit
CTCGTACACAGTGATGGAAACATTTACTTGCATTGGCTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATT11600.12432878532920331No Hit
GTGATGGAAACATTTACTTGCATTGGCTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTT11520.12347134543038124No Hit
CTCCCTGCCCGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACACAGTGA11310.12122056569597325No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTCGTTGCTATTTTAGAAGG11120.11918414593627077No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10820.11596874631568793No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10730.11500412642951308No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10700.11468258646745479No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA10670.11436104650539651No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG10670.11436104650539651No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10400.11146718684687194No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10160.10889486715040568No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACGCTGGTCCCTTGGCCGAACGTCCAAGGCCA10030.10750152731481978No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT9920.1063225474539394No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9880.10589382750452836No Hit
ATATGTGGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAA9870.10578664751717559No Hit
CAGTAATACACAGCCGTGTCCTCGACTTTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTC9850.10557228754247007No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9740.10439330768158969No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC9730.10428612769423694No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC9700.10396458773217865No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT9630.10321432782070931No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACATTTACTTGCATTGGCTTCAGCAGAGGCCAGGCCAATCTC9580.10267842788394552No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9550.10235688792188723No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9500.10182098798512342No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGTGATGTTGTGGTGACTCAGTCTCCAC9460.10139226803571237No Hit
GGGCTGGAGTGCGTGGCCAACATAAAGGAAGATGGAAGTGTGACACAATA9440.10117790806100685No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCC9390.10064200812424304No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9380.10053482813689028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)