FastQCFastQC Report
Wed 8 May 2024
EGAF00004717852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933010
Total Bases67.7 Mbp
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT33340.3573380778341068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28170.3019260243727291No Hit
GTAATACACAGCCGTGTCCTCGACTTTCAGGCTGTTCATTTGCAGATACA26370.28263362664923203No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25330.2714869079645449No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23910.25626734976045273No Hit
GTACACAGTGGCCTTGGACGTTCGGCCAAGGGACCAGCGTGGAAATCGAA22330.2393329117587164No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20550.22025487400992488No Hit
CCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA20540.22014769402257212No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG20420.21886153417433898No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20350.2181112742628696No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19470.20867943537582662No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT19010.2037491559575996No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18130.19431731707055658No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT18010.19303115722232345No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18010.19303115722232345No Hit
GTGTATTACTGTGCGAGAGGTCGTATAATTGGAATGACGGGAGGGGGAAC17560.18820805779144917No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17150.18381367830998596No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTG17090.18317059838586938No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16950.18167007856293074No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG16220.17384593948617913No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15890.170308999903538No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAAAAATTAG15480.1659146204220748No Hit
ACTGTGTACCTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACC15360.16462846057384165No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA15010.160877161016495No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGA14870.15937664119355632No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14550.15594688159826797No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA14550.15594688159826797No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14380.15412482181327103No Hit
GTGTACCTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACCCTG14290.15316020192709617No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14220.15240994201562685No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA14150.1516596821041575No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACGCTGGTCCCTTGGCCGAACGTC14100.15112378216739372No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC13940.14940890236974952No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13190.1413704033182924No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13130.14072732339417585No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13100.14040578343211754No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC13030.13965552352064822No Hit
GGGCTGGAGTGCGTGGCCAACATAAAGGAAGATGGAAGTGTGACACAATA12910.1383693636724151No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12870.13794064372300405No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12660.13568986398859603No Hit
CTCCCTGCCCGTCACCCTTGGGCAGCCGGCCTCCATCTCCTGCAGGTCTA12410.13301036430477703No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12360.13247446436801322No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCACGCTGGTCC12200.13075958457036901No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12020.1288303447980193No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGTGATGTTGTGGTGACTCAGT11770.12615084511420027No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11700.12540058520273095No Hit
CTCGTACACAGTGATGGAAACATTTACTTGCATTGGCTTCAGCAGAGGCC11680.12518622522802542No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGTGATGTTG11630.12465032529126162No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11530.12357852541773401No Hit
GTGTACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGCCCAA11500.12325698545567572No Hit
GTGATGGAAACATTTACTTGCATTGGCTTCAGCAGAGGCCAGGCCAATCT11390.12207800559479534No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGGCAGCCGGCCTCCATCTCCT11350.1216492856453843No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG11120.11918414593627077No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11110.11907696594891802No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC11110.11907696594891802No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC11060.11854106601215421No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11040.11832670603744869No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10750.1152184864042186No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10520.1127533466951051No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGT10380.11125282687216642No Hit
TTATTACTGTATGCAAGGTACACAGTGGCCTTGGACGTTCGGCCAAGGGA10380.11125282687216642No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10340.11082410692275539No Hit
CTGTGTACCTTGCATACAGTAATAAACCCCAACATCCTCAGCCTCCACCC10300.11039538697334435No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10260.1099666670239333No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCA10240.10975230704922775No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGT9950.1066440874159977No Hit
ATATGTGGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAACGCCA9890.10600100749188111No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT9870.10578664751717559No Hit
GGTCTAGTCAAAGCCTCGTACACAGTGATGGAAACATTTACTTGCATTGG9650.10342868779541484No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACTAATTTTTGGATGAGCTGGG9540.10224970793453446No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGTGA9480.1016066280104179No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA9340.10010610818747923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)