FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966618
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA33930.3510176719241727No Hit
GTATTACTGTCAGCAGTATCGTAGTTCACCGTGGACGTTCGGCCATGGGA28530.2951527904508296No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27760.2871868721666677No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC24020.24849526907216707No Hit
GTATATTACTGTGCGAGACATAAGCGGGAGCAACTGGCTCTAGGGGTCTT22400.23173580463016416No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT20710.21425216579869194No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC20620.21332108444080286No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC19420.2009066663356155No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC17910.18528519021992143No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17660.18269885311467404No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16710.17287077211473406No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15780.16324959808321385No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15660.16200815627269513No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15510.1604563540095467No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAACAGACACGTCCA15140.1566285750937806No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCCGCCAGGGAAGGG15130.1565251216095707No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14580.15083517997802648No Hit
GTGTATTACTGTCAGCAGTATCGTAGTTCACCGTGGACGTTCGGCCATGGGACCAAGGTGGAAATCAAACGA14030.14514523834648227No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13930.14411070350438332No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13910.14390379653596352No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13790.1426623547254448No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTTAGCGG13550.14017947110440732No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13530.13997256413598755No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA13460.1392483897465183No Hit
CTCCTGCAAGGCCAGTCAGAGTGTTAGCGGCAGCTACTTAGCCTGGTACC13210.1366620526412709No Hit
GTATCGTAGTTCACCGTGGACGTTCGGCCATGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT12840.1328342737255048No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT12710.13148937843077615No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12600.1303513901044673No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG12590.13024793662025744No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12420.12848922738868923No Hit
CTACGATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC12360.12786850648342987No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC12350.12776505299921997No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGAGTGTTAGCGGC12310.12735123906238038No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC12060.12476490195713302No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCATGGCCGAACGTCCACGGTGAACTACGATACTGCTGAC11950.12362691363082418No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11840.12248892530451533No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11630.12031640213610754No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11460.11855769290453934No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT11400.11793697199927998No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAAGGCCAGTCAGA11140.1152471814098227No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10950.1132815652098347No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC10900.11276429778878524No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC10760.1113159490098467No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10750.1112124955256368No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10630.10997105371511808No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG10440.10800543751513006No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGCGGCTGCCGGA10360.10717780964145092No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAACAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTG10240.10593636783093217No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGACCCCTAG10080.10428111208357387No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG10060.10407420511515408No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCCGCTAACACTCTG10030.10376384466252439No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGACCCCTAGAGCCAGTTGCTCCCGCTTATGTCTCGCACAGTAATATACGG9930.10272930982042545No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9800.10138441452569683No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9800.10138441452569683No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9740.10076369362043744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)