FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090849
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA45440.4165562786416818No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCT39200.3593531277014509No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAG35750.3277263855950732No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30900.2832656032136437No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29690.2721733255473489No Hit
GTGTATTACTGTGCGAATAGTTCGAGTATAGCAGCTCCCCGGGACCACTA29610.271439951817346No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28800.26401454280106595No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG26830.24595521469974305No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA23200.21267838170085868No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22870.20965321506459647No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT20690.18966878092201578No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20360.18664361428575357No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20290.18600191227200097No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC20270.18581856883950026No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC20230.18545188197449877No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19750.181051639594481No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC19640.18004325071572694No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG19390.17775145780946766No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19220.17619303863321137No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19030.17445127602445434No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18950.1737179022944514No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18530.16986769021193585No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT18520.16977601849568547No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGA18120.16610914984567066No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGCTGTCATCCCATGCTGCACAGTAAT17760.16280896806065734No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG17640.1617089074656529No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCAACCACTCAGGCTGTCATCCCATG17620.16152556403315216No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT17120.15694197822063363No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16930.1552002156118766No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16750.15355012471936996No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16490.15116666009686033No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC16220.14869152375810032No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTC16180.14832483689309886No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC16110.14768313487934628No Hit
CTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCT16040.14704143286559368No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16000.14667474600059222No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15860.145391341973087No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG15580.14282453391807665No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15500.1420911601880737No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTG15230.13961602384931368No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15000.1375075743755552No Hit
CCCCTGAGGGCCGCTGATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTAT15000.1375075743755552No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA14970.13723255922680408No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14740.13512410975304556No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14600.13384070572554038No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14490.1328323168467863No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14340.13145724110303075No Hit
GCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14230.13044885222427668No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG14140.12962380677802335No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14120.12944046334552262No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC13860.127056998723013No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTT13840.12687365529051225No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13810.12659864014176114No Hit
CTATTATAGTGGGAGCACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGA13760.1261402815605093No Hit
GATGTGTCCTGTCCCAGCTGCACCTGCAAGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACCCTGTCCCTC13690.1254985795467567No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA13670.12531523611425596No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTC13660.12522356439800558No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13610.12476520581675374No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13530.12403183208675077No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13340.12229006947799374No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCC13280.12174003918049153No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGA13180.12082332201798782No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12940.11862320082797893No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12910.11834818567922784No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12520.11477298874546339No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC12510.11468131702921303No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12460.11422295844796117No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12390.11358125643420858No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12300.11275621098795524No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12190.11174782210920117No Hit
CTATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTACT12120.1111061200954486No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCTTGCAGGTGC12040.11037274636544563No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12020.1101894029329449No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG12000.11000605950044415No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT11690.10716423629668267No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT11570.10606417570167824No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11560.10597250398542786No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11430.10478077167417306No Hit
GATTATTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG11430.10478077167417306No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11400.10450575652542193No Hit
GTATTACTGTGCGAATAGTTCGAGTATAGCAGCTCCCCGGGACCACTACTGGGGCCAGGGAACCCTGGTCACCGT11400.10450575652542193No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCC11300.10358903936291824No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11260.10322235249791677No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11220.10285566563291529No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGTGTC11130.10203062018666195No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11130.10203062018666195No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC11110.10184727675416122No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG11090.10166393332166046No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10940.10028885757790491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)