FastQCFastQC Report
Wed 8 May 2024
EGAF00004717861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610437
Total Bases117.9 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC99330.6167891075527947No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT91270.5667405803517928No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT77900.483719636347153No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC77120.4788762304889915No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC68410.42479153173952167No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC59140.3672295159636794No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56320.3497187409380187No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC53150.33003464277087524No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51050.3169947039219789No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG50050.31078520923202835No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49930.3100400698692342No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA48180.2991734541618207No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT44990.27936516610087825No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG43340.2691194998624597No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG42180.261916486022117No Hit
GTTATGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG41260.2562037509073624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT40970.25440299744727674No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA40940.2542167126065782No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG40020.24850397749182365No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG39740.2467653189786375No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA38280.23769945673130957No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37690.2340358548642387No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37400.23223510140415304No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC36860.2288819742715797No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC36830.2286956894308812No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCT36660.2276400753335896No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA35570.2208717261215434No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA34820.21621460510408047No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT34690.21540737079438688No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA34550.2145380415377938No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG34360.2133582375467032No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC33880.21037768009552685No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG32560.20218114710479204No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32180.1998215391226108No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT32170.19975944417571131No Hit
TCCCATAACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGC31960.19845545029082168No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC31880.19795869071562566No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29890.1856017962826239No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT29670.18423570745083478No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC29540.1834284731411412No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC29240.18156562473415602No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC29070.1805100106368644No Hit
CAGTATAATAGTTATGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAA29030.18026163084926639No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCG28680.17808830770778367No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT27610.1714441483895365No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC27570.17119576860193847No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG26870.16684912231897303No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA26640.1654209385402844No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAAGGCG26340.16355809013329922No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGC25400.15772116512474563No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC25100.15585831671776046No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA24660.15312613905418218No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24420.15163586032859405No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24260.15064234117820194No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23810.14784806856772417No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC23490.14586103026693997No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23300.14468122627584937No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23170.14387399196615577No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG22920.14232161829366813No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC22730.1411418143025775No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT22650.14064505472738145No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22590.1402724850459844No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22540.13996201031148686No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC22510.13977572547078837No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22430.13927896589559233No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA22370.1389063962141953No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC22260.1382233517983007No Hit
TTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC22260.1382233517983007No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC21720.13487022466572737No Hit
ACTTATTACTGCCAACAGTATAATAGTTATGGGACGTTCGGCCAAGGGAC21510.13356623078083776No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC21260.1320138571083501No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT20980.1302751985951639No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20850.12946796428547036No Hit
AGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGAC20600.1279155906129827No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATC20560.12766721082538465No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT20090.12474874832110788No Hit
GCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTA20020.12431408369281134No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19980.1240657039052133No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19910.12363103927691677No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19900.12356894433001725No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19740.12257542517962515No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGT19600.12170609592303207No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19590.12164400097613257No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC19570.12151981108233356No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19360.12021581719744392No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19280.11971905762224787No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGC19230.11940858288775034No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19170.11903601320635329No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA18900.11735944964006664No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC18600.11549660123308146No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18600.11549660123308146No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18520.1149998416578854No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTT18430.11444098713578985No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC18300.11363375282609627No Hit
GGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC18110.11245394883500565No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17980.11164671452531208No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17680.10978386611832688No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17460.10841777728653776No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17280.10730006824234664No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA17150.10649283393265306No Hit
GCTGATACCAGGCCAACCAGCTACTAATACTCTGACTGGCCCGGCAAGTG17060.1059339794105575No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA17050.105871884463658No Hit
CTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTAC17000.10556140972916048No Hit
GTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAA16960.10531302994156244No Hit
CCCCTAAGCTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA16940.10518884004776342No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16890.1048783653132659No Hit
GATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGC16590.10301551690628072No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16540.1027050421717832No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA16510.10251875733108466No Hit
GCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC16420.1019599028089891No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16120.10009705440200392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)