FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485564
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTATTACTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTCATCTATCTGGCAT13930.2868828825860237No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13630.28070450033363264No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT12930.2662882750780535No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9860.2030628300285853No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA9530.19626660955095518No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9370.19297147234967998No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG9200.18947038907332503No Hit
CTATTATTGTGTGACAGATAAGAAGTACGGATATCACCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC8760.18040876176981818No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTAGTA8690.17896713924426028No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG8580.17670173241838358No Hit
CCTTCCACCCTGTCTGCATCTGTAGGTGACAGGGTCACCATCACTTGCCG8250.16990551194075343No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7780.16022604641200747No Hit
CAATAATAGAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA7770.1600201003369278No Hit
GTATATGGTGATACTACTACCACTAATGTATGAAATCCACTCCAGCCCCTTCCCCGGAACCTGCCGTACCCAGTT7750.15960820818676838No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT7590.15631307098549316No Hit
GTCTGCATCTGTAGGTGACAGGGTCACCATCACTTGCCGGGCCAGTCAGA7410.15260604163405853No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7290.15013468873310212No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTA7180.1478692819072254No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA7140.1470454976069066No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7060.145397929006269No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6860.14127900750467498No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG6850.14107306142959528No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC6850.14107306142959528No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTCCGAGAATAAGTATGATATTGTTGGC6710.13818981637847946No Hit
ATATCATACTTATTCTCGGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT6660.13716008600308097No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6590.13571846347752303No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT6580.13551251740244336No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC6550.13489467917720424No Hit
ACCTTGAAGGGACCCCACTTTGTAAAGTAGATGCCAGATAGATGAGGACCTTAGGGGCTTTCCCTGGTTTCTG6510.13407089487688545No Hit
CTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG6400.13180548805100872No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6370.1311876498257696No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCCGAGACAACGCCGAGAACTCATTGTTTCTG6270.12912818907497262No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6200.1276865665494147No Hit
GTATGATATTGTTGGCAGTAGTAAGTCGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC6160.12686278224909592No Hit
ATAATAGAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAATGAGTTCTCGGCGTTGTCTCGGG6090.12542115972353798No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5960.12274386074750188No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5930.12212602252226278No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTCCGAGAATAAGT5890.12130223822194397No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC5850.12047845392162516No Hit
CCCCTAAGGTCCTCATCTATCTGGCATCTACTTTACAAAGTGGGGTCCCT5830.12006656177146577No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC5830.12006656177146577No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5820.11986061569638606No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5770.11883088532098755No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5650.11635953242003115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5590.11512385596955292No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAA5470.11265250306859652No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG5340.10997520409256041No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC5320.10956331194240101No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA5300.10915141979224159No Hit
GTAATACTCTGACTGGCCCGGCAAGTGATGGTGACCCTGTCACCTACAGA5290.1089454737171619No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC5280.1087395276420822No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5210.1072979051165243No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG4970.10235519931461146No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC4910.10111952286413325No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGTGACAGGGTCACCATCACTTGCCGGGCCAGTCAGAGTATTACTA4890.10070763071397386No Hit
ACTTTACAAAGTGGGGTCCCTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC4870.10029573856381445No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC4860.10008979248873474No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)