FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485564
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13200.27184881910520553No Hit
GAGTATTACTAACTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTCATCTATCTGGCAT10920.22489311398703363No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT9870.203268776103665No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA8960.18452768327141222No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA8630.17773146279378207No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8460.17423037951742718No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8460.17423037951742718No Hit
CTATTATTGTGTGACAGATAAGAAGTACGGATATCACCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC8290.17072929624107222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8000.1647568600637609No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7910.16290334538804357No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGTTAGTA7800.16063793856216688No Hit
CAATAATAGAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA7490.15425361023469614No Hit
GTATATGGTGATACTACTACCACTAATGTATGAAATCCACTCCAGCCCCTTCCCCGGAACCTGCCGTACCCAGTT7260.14951685050786304No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT7230.14889901228262392No Hit
GTATGATATTGTTGGCAGTAGTAAGTCGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC6940.14292657610531256No Hit
ATATCATACTTATTCTCGGACATTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT6880.1416908996548344No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC6850.14107306142959528No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG6770.13942549282895766No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6640.13674819385292156No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6480.13345305665164633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6470.13324711057656663No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG6380.13139359590084934No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCGACTTA6320.1301579194503711No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6160.12686278224909592No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCCGAGACAACGCCGAGAACTCATTGTTTCTG6120.12603899794877707No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6100.1256271057986177No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTCCGAGAATAAGTATGATATTGTTGGC6070.1250092675733786No Hit
GTCTGCATCTGTAGGTGACAGGGTCACCATCACTTGCCGGGCCAGTCAGA5980.12315575289766129No Hit
CCTTCCACCCTGTCTGCATCTGTAGGTGACAGGGTCACCATCACTTGCCG5960.12274386074750188No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAATGTCCGAGAATAAGT5820.11986061569638606No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT5730.11800710102066875No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC5680.11697737064527024No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC5630.11594764026987174No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT5590.11512385596955292No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG5590.11512385596955292No Hit
ATAATAGAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAATGAGTTCTCGGCGTTGTCTCGGG5580.11491790989447323No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5560.11450601774431383No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5390.11100493446795891No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGAAGGGACCCCACTTTGTAAAGTAGATGCCAGAT5370.1105930423177995No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC5320.10956331194240101No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT5290.1089454737171619No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5220.107503851191604No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5200.10709195904144458No Hit
ACCTTGAAGGGACCCCACTTTGTAAAGTAGATGCCAGATAGATGAGGACCTTAGGGGCTTTCCCTGGTTTCTG5110.10523844436572728No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCC5090.10482655221556787No Hit
CTTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG4940.10173736108937237No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT4920.10132546893921296No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4910.10111952286413325No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG4870.10029573856381445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)