FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865075
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33090.3825101869780077No Hit
GACCTAGGGCGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGA30080.3477155159957229No Hit
GCCTTGGGCTGACCTAGGGCGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGACTGTCATCCCAGG29650.3427448487125394No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG29120.33661821229373173No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG24060.27812617403115336No Hit
GTAATACACGGCCGTGTCTGCAGCGGTCACTGAGTTCAGCTTCAGGGAGAACTGGTTCTTGAACAAGTCTACTGA22450.2595150709476057No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22000.2543132098372973No Hit
GTCCTGGGCCCAGTCTGTGGTGGCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTG20840.24090396786405804No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGGCGGTCAGCTTGGTCCCTC20330.2350085252723752No Hit
GTCTGTGGTGGCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG19170.2215992832991359No Hit
ATAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG19060.22032771724994943No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGGTGGCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCA18300.21154235181920644No Hit
GACTATTATTGTGCAGCCTGGGATGACAGTCTGAGTGGTTGGGTGTTCGG17810.20587810305464846No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17560.20298818021558823No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC17390.2010230326850273No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17380.20090743577146492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17200.19882669132734154No Hit
GTCTCTGGTGGCTCCATCAGTAGCTACTACTGGACCTGGATCCGGCAGGCCCCAGGGAAGGGACTGGAGTGGAT16680.19281565182209634No Hit
CTATTATTGTGCAGCCTGGGATGACAGTCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGCCCTAGG16270.18807617836603763No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16240.18772938762535038No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG16170.18692020923041355No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16010.185070658613415No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15190.1755917117012976No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14780.17085223824523885No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14700.16992746293673958No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14210.16426321417218162No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGGCTCAGCCAC13990.16172008207380864No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13610.15732739935843712No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13330.15409068577868973No Hit
CCCCTGAGGGCCGCTGACTATTCCTATAGATGAGGAGTTTGGGGGCCGTTGCTGGGAACTGCTGGTACCAGTATA13240.15305031355662804No Hit
CAATAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA13180.15235673207525358No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13090.15131635985319192No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12960.14981359997688062No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12850.14854203392769413No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG12560.1451897234343843No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12360.14287778516313612No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT12360.14287778516313612No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12050.1392942808427015No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11930.1379071178799526No Hit
GTGGTGGCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11920.13779152096639022No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA11830.13675114874432853No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11560.13363003207814353No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGACTA11530.13328324133745628No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11450.132358466028957No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11420.13201167528826982No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT11360.13131809380689535No Hit
ATCCATAACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT11320.13085570615264572No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11310.13074010923908333No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT11150.1288905586220848No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG11010.12727220183221108No Hit
GTGTATTACTGTGCGAGAGTTGATGTTACTATGATAGAACCGTGGGGCCA10950.12657862035083664No Hit
GCCTGGGATGACAGTCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGCCCTAGGTCAGCCCAAGG10930.1263474265237118No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10900.1260006357830246No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10840.12530705430165015No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10430.12056758084559142No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCC10350.11964280553709217No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10320.11929601479640493No Hit
GACTATTCCTATAGATGAGGAGTTTGGGGGCCGTTGCTGGGAACTGCTGGTACCAGTATACAAAATTACTTCCGA10250.11848683640146808No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGACTATTATTGTGCAGCCTGGGATGACAGTCTGAGTGGT10180.11767765800653122No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAAT10140.1172152703522816No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTG10090.11663728578446957No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGGCGGTCAGCTTGGTCCCTCCGCCGAACACCCAAC9690.11201340924197323No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGG9660.11166661850128602No Hit
CATCTGCACTGTCTCTGGTGGCTCCATCAGTAGCTACTACTGGACCTGGATCCGGCAGGCCCCAGGGAAGGGA9620.11120423084703637No Hit
GTTCTATCATAGTAACATCAACTCTCGCACAGTAATACACGGCCGTGTCT9600.11097303701991157No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGG9560.11051064936566193No Hit
ATCCCAGGCTGCACAATAATAGTCAGCCTCATCCTCGGACCGGAGCCCAC9500.10981706788428748No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9280.10727393578591451No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG9220.10658035430454006No Hit
CAGTAATACACGGCCGTGTCTGCAGCGGTCACTGAGTTCAGCTTCAGGGAGAACTGGTTCTTGAACAAGTCTA9210.10646475739097766No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9200.10634916047741526No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT9190.10623356356385286No Hit
GCTTGGTCCCTCCGCCGAACACCCAACCACTCAGACTGTCATCCCAGGCTGCACAATAATAGTCAGCCTCATCC9190.10623356356385286No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9160.10588677282316562No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG9130.1055399820824784No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8960.10357483455191746No Hit
GTTATGGATATGCCCAATCCACTCCAGTCCCTTCCCTGGGGCCTGCCGGATCCAGGTCCAGTAGTAGCTACTGA8950.10345923763835506No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8850.10230326850273097No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGACTATTCCTATAGATGAGGAGTT8800.10172528393491893No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC8770.10137849319423171No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGACTATTATTGTGCAGCC8690.10045371788573246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)