FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865075
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT30780.35580729994509147No Hit
CCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG28670.3314163511834234No Hit
GTCCTGGGCCCAGTCTGTGGTGGCTCAGCCACCCTCAGCGTCTGGGACCC28330.3274860561223015No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24130.2789353524260902No Hit
GTCTGTGGTGGCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCA23940.2767390110684045No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22020.25454440366442216No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGGTGGCTCAGCCACCCTCAGCGTCT20830.2407883709504956No Hit
GTAATACACGGCCGTGTCTGCAGCGGTCACTGAGTTCAGCTTCAGGGAGAACTGGTTCTTGAACAAGTCTACT20070.23200300551975261No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGGTGGCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCA19470.22506719070600817No Hit
GCCTTGGGCTGACCTAGGGCGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGACTGTCATCCCAG19160.22148368638557353No Hit
GACCTAGGGCGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCACTCAGA18960.21917174811432535No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17200.19882669132734154No Hit
GTCTCTGGTGGCTCCATCAGTAGCTACTACTGGACCTGGATCCGGCAGGCCCCAGGGAAGGGACTGGAGTGGATT17040.19697714071034303No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA16560.19142848885934743No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCG16370.18923214750166173No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA16100.1861110308354767No Hit
GACTATTATTGTGCAGCCTGGGATGACAGTCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGCCCTA15980.1847238678727278No Hit
CTATTATTGTGCAGCCTGGGATGACAGTCTGAGTGGTTGGGTGTTCGGCG15980.1847238678727278No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15940.18426148021847819No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15710.1816027512065428No Hit
GTGGTGGCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15450.17859723145392017No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGGCGGTCAGCTTGGTCCCTC15430.17836603762679537No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG15130.17489813021992312No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG14940.1727017888622374No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14930.17258619194867497No Hit
CCCCTGAGGGCCGCTGACTATTCCTATAGATGAGGAGTTTGGGGGCCGTTGCTGGGAACTGCTGGTACCAGTATA13870.16033291911105974No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA13450.1554778487414386No Hit
ATCCATAACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCACCATATCAGTAGACTTGTTCAA13380.15466867034650172No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13280.15351270121087768No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT13010.15039158454469267No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT12520.14472733578013466No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12440.14380256047163542No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG12110.13998786232407592No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11970.13836950553420224No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11950.1381383117070774No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11750.13582637343582926No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG11530.13328324133745628No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA11420.13201167528826982No Hit
GTGTATTACTGTGCGAGAGTTGATGTTACTATGATAGAACCGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCA11270.13027772158483367No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG11260.13016212467127128No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11200.1294685431898968No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTATACTGGTACCAGCAGTTCCCAGCAACGGCCCC11030.1275033956593359No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA10900.1260006357830246No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT10870.12565384504233737No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC10690.12357310059821403No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10680.12345750368465162No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT10520.12160795306765308No Hit
GCCTGGGATGACAGTCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGCCCTAGGTCAGCCCAAG10410.1203363870184666No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10390.12010519319134177No Hit
GACTATTCCTATAGATGAGGAGTTTGGGGGCCGTTGCTGGGAACTGCTGGTACCAGTATACAAAATTACTTCC10220.11814004566078086No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGG10160.1174464641794064No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT10160.1174464641794064No Hit
CATCTGCACTGTCTCTGGTGGCTCCATCAGTAGCTACTACTGGACCTGGATCCGGCAGGCCCCAGGGAAGG10100.11675288269803197No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTA9970.11525012282172066No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGACTATTATTGTGCAGCCTG9740.11259139380978528No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTTTGTATACTGGTACCAGCAGTTCCCA9640.1114354246741612No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9580.11074184319278677No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG9550.11039505245209952No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA9500.10981706788428748No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGACTATTATTGTGCAGCCTGGGATGACAGTCTGAGTGGTTG9430.10900788948935063No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC9370.10831430800797617No Hit
GGAATAGTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9330.10785192035372657No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGG9320.10773632344016415No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9260.10704274195878971No Hit
GTTCTATCATAGTAACATCAACTCTCGCACAGTAATACACGGCCGTGTCTGCAGCGGTCACTGAGTTCAGCTTCA9240.10681154813166488No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9150.10577117590960322No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG9140.1056555789960408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9080.10496199751466637No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG9040.10449960986041672No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT8970.10369043146547988No Hit
GTTTGGGGGCCGTTGCTGGGAACTGCTGGTACCAGTATACAAAATTACTT8870.1025344623298558No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGACTATTCCTATAGATGAGGAGTTTG8800.10172528393491893No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8770.10137849319423171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)