FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138208
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51780.4549256374933228No Hit
ATTTTACACAGCTCCAACAATAAGAACTTCTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACTACTC51270.45044490989344654No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT48820.4289198459332565No Hit
ATATTATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46300.4067797801456325No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTAAGAAGTTCTTATTGTTGGAGCTGTGTAAAATACT36960.3247209648851528No Hit
CTCCTAAACTACTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCCCT36790.3232273890185273No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35880.31523236526188536No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34970.3072373415052433No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG34530.303371615732801No Hit
ATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA34350.3017901824622565No Hit
TTATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT33130.291071579184121No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG30640.26919508560825434No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAATCTGTC30020.2637479265652675No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29620.2602336304085018No Hit
GTGTAAAATACTCTGGCTGGACTTGCACTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGG28640.2516236048244258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT28210.2478457364559026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA27930.24538572914616663No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAAT26850.23589712952289915No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC25910.2276385335544997No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25790.22658424470747No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG25640.22526638364868284No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTAC24990.21955565239393854No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC24680.2168320728724451No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24120.2119120582529731No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA24030.2111213416177008No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23330.2049713233433608No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAAT22870.20092988276308021No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22390.19671272737496137No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCGCCAGCC21880.19223199977508504No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA21220.18643341111642162No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20950.18406126121060473No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGTACTATAATATTGCTGAC20300.17835052995586043No Hit
GCTCCAACAATAAGAACTTCTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACTACTCATTTACTGGG20080.1764176670696393No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC19820.17413337456774158No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTACACAGCTCCA19590.17211265427760128No Hit
AACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTGCCCGGGAA19090.16771978408164412No Hit
GTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTACACAGCTCCAACAATAAGAACTT18960.16657763783069526No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18340.1611304787877084No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18260.16042761955635526No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT18050.15858261407405325No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC17940.15761618263094268No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTACACAGCTCCAACA17860.15691332339958952No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC17530.1540140290702578No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17430.15313545503106638No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17130.15049973291349208No Hit
GTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC16860.1481275830076752No Hit
GTGCAAGTCCAGCCAGAGTATTTTACACAGCTCCAACAATAAGAACTTCTTAGCTTGGTTCCAACAGAAACCAGG16750.14716115156456464No Hit
GTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTA16670.1464582923332115No Hit
GCCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGT16620.14601900531361578No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16080.14127470550198207No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16050.14101113329022463No Hit
GATTTCACTCTCACCATCGCCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT16010.14065970367454805No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15850.13925398521184176No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15700.13793612415305462No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT15690.13784826674913547No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT15630.13732112232562063No Hit
CAGTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGCTGATGGACCTGTCGG15170.13327968174534002No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15160.1331918243414209No Hit
GTCCTGGTTTCTGTTGGAACCAAGCTAAGAAGTTCTTATTGTTGGAGCTGTGTAAAATACTCTGGCTGGACTTGC15140.13301610953358262No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14220.12493322837302145No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT14140.1242303691416683No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13900.12212179144760887No Hit
GTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGA13700.120364643369226No Hit
GAATCGGTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTT13690.12027678596530687No Hit
CTACGGGGACATCGTGGTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTG13650.1199253563496303No Hit
GTATTTTACACAGCTCCAACAATAAGAACTTCTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACT13580.1193103545221963No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA13450.11816820827124744No Hit
ACTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13410.11781677865557086No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA13300.11685034721246029No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGGTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13280.11667463240462199No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13240.11632320278894544No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC13230.11623534538502628No Hit
GGTCTGGGACAGATTTCACTCTCACCATCGCCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCAGCAAT13110.11518105653799658No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA13070.11482962692232No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12810.11254533442042229No Hit
GTCTGGGACAGATTTCACTCTCACCATCGCCAGCCTGCAGCCTGAAGATG12800.11245747701650313No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12700.1115789029773117No Hit
TCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC12640.11105175855379684No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12550.11026104191852455No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12540.11017318451460542No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAAT12500.10982175489892884No Hit
CACCATCGCCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGTGGACGTT12470.10955818268717141No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12400.1089431808597374No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12210.1072738901852737No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12190.1070981753774354No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12180.10701031797351628No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12100.1063074587421631No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12080.10613174393432484No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTC12060.10595602912648655No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGGTG11930.1048138828755377No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCAC11890.10446245325986112No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11870.10428673845202283No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATC11730.10305673479715483No Hit
CTTCTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTGCCCGGGAATC11650.1023538755658017No Hit
TTCCCGGGCAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTAAGAAGTT11630.1021781607579634No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11570.10165101633444852No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11440.10050887008349967No Hit
GTATTACTGTGCGCGACCGGGGGCAGGCTATGATAACGTTTGGGGGAGTCATCGTCGCCTTGACGCTTTTGATAT11420.10033315527566138No Hit
ATTATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA11400.10015744046782311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)