FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138208
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52320.45966993730495653No Hit
ATATTATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT46070.4047590598554921No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT41520.364783941072282No Hit
ATTTTACACAGCTCCAACAATAAGAACTTCTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACTACTC39290.34519173999831315No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAATCTGTC37280.32753240181056537No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35470.3116302117012005No Hit
GTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAAT35370.31075163766200903No Hit
GTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTAAGAAGTTCTTATTGTTGGAGCTGTGTAAAATACT33420.29361944389777617No Hit
TTATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT31570.2773658241727347No Hit
ATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA31430.27613582051786667No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31030.27262152436110093No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG30940.2718308077258287No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA30220.26550507464365036No Hit
CTCCTAAACTACTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCCCT29570.25979434338890606No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29340.2577736230987658No Hit
GAGTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAAT27910.2452100143383283No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT26930.2365999887542523No Hit
GTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC25340.22263066153110855No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAATGAGTAGTTT24850.2183256487390705No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC23620.20751918805701594No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23600.20734347324917768No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG23380.2054106103629565No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23210.20391703449633108No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22130.19442843487306363No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG21730.1909141387162979No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGTACTATAATATTGCTGAC21720.19082628131237875No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21620.18994770727318733No Hit
GTTTATTACTGTCAGCAATATTATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA21590.1896841350614299No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21460.18854198881048104No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC21060.1850276926537153No Hit
GTGTAAAATACTCTGGCTGGACTTGCACTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGG20520.18028339284208159No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTAC20170.17720838370491157No Hit
TCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC19970.17545123562652873No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19210.16877407292867386No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA19120.16798335629340155No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC18430.1619211954229807No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT18430.1619211954229807No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC18240.16025190474851697No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCGCCAGCC17720.15568331974472155No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17540.15410188647417694No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG17400.15287188281930894No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17390.15278402541538982No Hit
GTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGA16320.1433832831960415No Hit
GCCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCGT16130.1417139925215778No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC15970.14030827405887147No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTACACAGCTCCA15820.13899041300008433No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT15660.13758469453737807No Hit
GTAAATGAGTAGTTTAGGAGGCTGTCCTGGTTTCTGTTGGAACCAAGCTA15620.1372332649217015No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15400.13530040203548035No Hit
AACCAGGACAGCCTCCTAAACTACTCATTTACTGGGCATCTGCCCGGGAA15310.13450968540020805No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15150.13310396693750176No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15000.13178610587871462No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC14860.13055610222384662No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14620.1284475245297872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14590.12818395231802973No Hit
CAGTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTGCTGATGGACCTGTCGG14570.12800823751019147No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14420.12669037645140432No Hit
GCTCCAACAATAAGAACTTCTTAGCTTGGTTCCAACAGAAACCAGGACAGCCTCCTAAACTACTCATTTACTGGG14280.1254603727965363No Hit
GTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTACACAGCTCCAACAATAAGAACTT13870.12185821923585145No Hit
CAGTAATAAACTGCCACATCTTCAGGCTGCAGGCTGGCGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCC13870.12185821923585145No Hit
GATTTCACTCTCACCATCGCCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACT13810.12133107481233657No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAAGTGCAAGTCCAGCCAGAGTATTTTACACAGCTCCAACA13810.12133107481233657No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13540.11895892490651973No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC13490.11851963788692402No Hit
GTCCTGGTTTCTGTTGGAACCAAGCTAAGAAGTTCTTATTGTTGGAGCTGTGTAAAATACTCTGGCTGGACTTGC13300.11685034721246029No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12950.11377533807529028No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12780.11228176220866484No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA12570.11043675672636284No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACG12500.10982175489892884No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12490.1097338974950097No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12460.10947032528325228No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG12400.1089431808597374No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGT12370.10867960864797997No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGA12310.10815246422446513No Hit
GTGCAAGTCCAGCCAGAGTATTTTACACAGCTCCAACAATAAGAACTTCTTAGCTTGGTTCCAACAGAAACCAGG12310.10815246422446513No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTACGGGGACATCGTGGTG12280.10788889201270771No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12230.10744960499311199No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGGTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG12220.10736174758919283No Hit
GTCTGGGACAGATTTCACTCTCACCATCGCCAGCCTGCAGCCTGAAGATG12060.10595602912648655No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12030.10569245691472913No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11900.10455031066378027No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11880.10437459585594197No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11870.10428673845202283No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGA11810.10375959402850797No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11690.10270530518147825No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11670.10252959037363996No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAAT11510.10112387191093368No Hit
ATTATAGTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA11490.10094815710309539No Hit
GGTCTGGGACAGATTTCACTCTCACCATCGCCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTACTGTCAGCAAT11470.1007724422952571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)