FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007867
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAGCTCTCAGGCTGTTCAGTTGAAGATACAGCGTGTTCTTGGAATTGTCTCTGG49970.4957995449796452No Hit
GTATATTACTGTGCGAGAGATTGGGCTCCGGTGACTAGGAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTC23460.23276880778912296No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23270.2308836384165768No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT21140.2097498975559275No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21080.20915458091196557No Hit
CTCTACCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTACTGAAGGTGAATCCAGAGGCTGCAC19590.19437088425357713No Hit
CTGTAAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC18850.18702864564471303No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18370.1822661124930174No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18300.18157157640839516No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCC17270.17135197402038166No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17070.16936758520717515No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTCTGCTGCATC16900.16768085471594962No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16850.167184757512648No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTG16200.16073549386972685No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCC15900.1577589106499171No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15720.15597296071803124No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15540.1541870107861454No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15250.15130964700699595No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG15000.14882916099048785No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG14860.14744008882124326No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14760.14644789441464004No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14580.14466194448275416No Hit
GGCTTACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT14200.1408916057376618No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGGT14050.13940331412775694No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14040.13930409468709662No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGGTAGAGTGGGTGGCAGTTATATCATATGATGGGGGTAAT13820.13712126699256943No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13450.13345014768813743No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13360.1325571727221945No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG13250.13146575887493092No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13240.1313665394342706No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13100.12997746726502604No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC12960.1285883950957815No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTG12950.12848917565512116No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12890.1278938590111592No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12800.12700088404521628No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAA12800.12700088404521628No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT12590.12491727579134945No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12520.12422273970672718No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12420.12323054530012394No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12360.12263522865616197No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC12210.12114693704625709No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG12140.12045240096163481No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11800.11707893997918377No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA11630.11539220948795824No Hit
GTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11530.11440001508135499No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTT11480.11390391787805335No Hit
GCCCAGCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAAC11470.11380469843739302No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCACCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGAAGTCCCTG11270.11182030962418653No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC11100.11013357913296098No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11040.10953826248899905No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA11020.1093398236076784No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11000.10914138472635775No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10990.10904216528569742No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10980.10894294584503711No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG10660.10576792374390669No Hit
CAGTAATATACAGCCGTGTCCTCAGCTCTCAGGCTGTTCAGTTGAAGATA10650.10566870430324636No Hit
GGCCAAAAGTGTACGGGAAACTGTAAGCCTGTTGACAATAGTAAGTTGCA10570.10487494877796374No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT10430.1034858766087192No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGT10400.10318821828673823No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA10350.10269212108343662No Hit
GTGCATAGCATAGCTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC10320.10239446276145561No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG10300.10219602388013498No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG10270.101898365558154No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG10220.10140226835485239No Hit
ATCTTCAACTGAACAGCCTGAGAGCTGAGGACACGGCTGTATATTACTGTGCGAGAGATTGGGCTCCGGTGACT10090.10011241562626816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)