FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314738
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49460.3761966262479673No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTTTC40950.31146890102818964No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT38780.2949637114010548No Hit
GTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC35800.2722975984568789No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC33960.25830241462557557No Hit
GCGTAGCACCTGGCTCGCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT32970.2507723972380809No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32690.24864269535070865No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT31320.23822236825892304No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT29800.22666112944175948No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29250.2224777864487069No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA28340.21555625531474712No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCT28220.21464352593444475No Hit
GTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGT27400.20840654183571175No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27090.20604865760326393No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG26920.20475562431450223No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT24980.18999983266628026No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAGCTGGTTCTTCGACGTGTCTACTG24850.18901104250428602No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACC23990.18246981527878559No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT22880.1740270685109885No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG22290.16953948239116842No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22190.16877887457424975No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC21830.1660406864333426No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGCGCGAGCCAGGTGCTACGCTG21140.16079249249660388No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20480.15577248090494075No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20440.15546823777817328No Hit
CTGCTACTCTGGCTCCCAGTTTCAGATACCACCGGAGAAATTGTGTTGAC20300.15440338683448718No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19760.15029610462312645No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19600.14907913211605658No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC19570.14885094977098098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18950.14413518130608532No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGAGCGCGAGCC18820.14314639114409106No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17840.13569243453828825No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT17430.13257394248892174No Hit
TCTATGATTCGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17320.13173727389031123No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17110.13013999747478203No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC17090.12998787591139832No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGACGAATCAT17070.1298357543480146No Hit
GTTTATTACTGTCAGCAGCGTAGCACCTGGCTCGCGCTCACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGA17050.12968363278463085No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17030.12953151122124712No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC16680.12686938386203184No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC16570.1260327152634213No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCACCTGG16550.12588059370003757No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA16450.12511998588311893No Hit
CTCCCAGGCTCCTCATCTATGATTCGTCCAACAGGGCCACTGGCATCCCA16050.1220775546154443No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCCGGAGACAAAGACAGGGTGGCTGG15720.11956754881961273No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTC15410.1172096645871649No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTC15310.11644905677024624No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC15090.1147757195730252No Hit
GTGTATTACTGTGCGAGCGCTCAACACGGTAATTTGAAATATTTCCACTATTATGGTGTGGACGTCTGGGGCCA15080.11469965879133331No Hit
GCCTGGGAGCCTGGCCAGGTTTTTGTTGGTACCAGGCTAAGTAGCTGCTAACACTCTGACTGGCCCTGCAGGAG14880.11317844315749603No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATTCGTCCA14840.11287420003072855No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14650.11142904517858311No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATT14500.11028813345320511No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC14390.10945146485459462No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGTTTC14340.10907116094613528No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTC14120.10739782374891423No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13880.10557236498830945No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTGAGCGCGAGCCAGGTGCTACGCTGCTGACAGTAATAAACTGCAAAAT13710.10427933169954774No Hit
CATCTATACCAGTGGGAGCACCAACTACAACCCCTCCCTCAAGGGTCGAGTCACCATGTCAGTAGACACGTCGA13670.10397508857278029No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAT13540.10298629841078602No Hit
ACTTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCAGGCTCCTCATC13250.10078053574172191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)