FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743593
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT20570.2766298230349129No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18990.25538164022523074No Hit
GGATATTAGTACCTGGCTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTGCTGCAT14840.19957153980739464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13790.1854509119908337No Hit
GGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCCATTTTAAAAGGTGTCC13390.1800716252035724No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13130.17657508879185255No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGGTCC13130.17657508879185255No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13120.176440606622171No Hit
CTATTATTGTCAACAGGCTAGCAGTTTCCCTTACACTTTCGGCGGAGGGA12700.1707923554955466No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA12530.16850615861096055No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG12090.1625889431449731No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG11690.1572096563577118No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT11580.15573035249121495No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCATCCTATTATTGTCAACAGGCTAGCAGTTTC11500.1546544951337627No Hit
GGCTAGCAGTTTCCCTTACACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT11480.15438553079439962No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10860.14604763627414458No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT10530.14160972467465402No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10320.13878559911134183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10190.1370373309054819No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC9980.1342132053421697No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG9920.1334063123240805No Hit
CAATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA9880.1328683836453544No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9440.12695116817936694No Hit
CTGCTAGCCTGTTGACAATAATAGGATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC9430.1268166860096854No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9370.1260097929915962No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9310.12520289997350703No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9250.1243960069554178No Hit
CTCCTGTGTAGGGTCTGGATTCACGTTTAGCAACCATGCCATGAGATGGGTCCGCCAGGCTCCAGGGAAGGGG9180.12345463176764708No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9090.1222442922405133No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA9060.12184084573146871No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8990.12089947054369796No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGGTCCAGCTGCGACATCC8920.11995809535592723No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8870.11928568450751957No Hit
ACCTTAGTGGGACCCCACTTTGCAAAAAAGATGCAGCAAAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG8810.11847879148943039No Hit
CAATAATAGGATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCGGATCCACTGCCG8790.1182098271500673No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGTAAGGGAAACTGCTAGCCTGTTGAC8690.11686500545325199No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8620.11592363026548126No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8570.1152512194170736No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC8450.1136374333808952No Hit
GATCAGGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGC8420.1132339868718506No Hit
CTAATATCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCCACAGT8240.110813307817583No Hit
GTGTAGGGTCTGGATTCACGTTTAGCAACCATGCCATGAGATGGGTCCGC8240.110813307817583No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8080.10866159310267848No Hit
GTGTAAGGGAAACTGCTAGCCTGTTGACAATAATAGGATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAG8030.10798918225427082No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7930.10664436055745549No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC7840.10543402103032169No Hit
GAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT7770.10449264584255097No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7770.10449264584255097No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGGTCCAGCTGCGACAT7730.10395471716382483No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC7600.10220644895796491No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTCGGAATTGTCTCTGG7570.10180300244892031No Hit
ATATTATTGTGCCCGGGGTGGAGTGAGCCGTTGGGACTACAACTACGGAA7450.10018921641274192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)