FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743593
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19190.2580712836188614No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT17090.2298300279857395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13400.1802061073732539No Hit
GGATATTAGTACCTGGCTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGATCTTTGCTGCAT12720.1710613198349097No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG12060.16218549663592852No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA11840.1592268889029348No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGGTCC11820.15895792456357172No Hit
CTATTATTGTCAACAGGCTAGCAGTTTCCCTTACACTTTCGGCGGAGGGA11720.15761310286675642No Hit
GGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCCATTTTAAAAGGTGTCC11530.15505794164280728No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11240.15115795872204285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11230.1510234765523613No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCATCCTATTATTGTCAACAGGCTAGCAGTTTC10860.14604763627414458No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT10290.13838215260229722No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10290.13838215260229722No Hit
CTGCTAGCCTGTTGACAATAATAGGATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC10150.13649940222675577No Hit
GGCTAGCAGTTTCCCTTACACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT9810.13192700845758365No Hit
GAGTGAACTCTGTCCCGGATCCACTGCCGCTGAACCTTAGTGGGACCCCACTTTGCAAAAAAGATGCAGCAA9620.12937184723363454No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC9600.12910288289427146No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9550.1284304720458638No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9440.12695116817936694No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT9290.12493393563414396No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG9040.12157188139210563No Hit
CTCCTGTGTAGGGTCTGGATTCACGTTTAGCAACCATGCCATGAGATGGGTCCGCCAGGCTCCAGGGAAGGGG9030.12143739922242408No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8910.11982361318624571No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC8840.11888223799847498No Hit
CAATAATAGGATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCGGATCCACTGCCG8840.11888223799847498No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGTAAGGGAAACTGCTAGCCTGTTGAC8780.11807534498038577No Hit
CAATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA8730.11740293413197811No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG8600.1156546659261182No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8540.11484777290802899No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8480.11404087988993979No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG8470.11390639772025826No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG8360.1124270938537614No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG8340.11215812951439834No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA8140.10946848612076769No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8040.10812366442395234No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC7910.10637539621809243No Hit
GTGTAGGGTCTGGATTCACGTTTAGCAACCATGCCATGAGATGGGTCCGC7830.10529953886064015No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGGTCCAGCTGCGACATCC7830.10529953886064015No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG7790.10476161018191403No Hit
GTGTAAGGGAAACTGCTAGCCTGTTGACAATAATAGGATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAG7780.10462712801223249No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7730.10395471716382483No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7650.10287885980637258No Hit
ACCTTAGTGGGACCCCACTTTGCAAAAAAGATGCAGCAAAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTTG7550.10153403810955725No Hit
GATCAGGACTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGC7490.10072714509146805No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGTGTTCTCGGAATTGTCTCTGG7470.10045818075210498No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTGTAAGGGAA7460.10032369858242346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)