FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339499
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCAGTCAATTTGCAAAGTGGGGTCCCACGAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTC44060.328928950301568No Hit
GTAGTAGACAGCCGTGTCCTCGCCTCTCAGGTCGTTCAATTGCAACCACACTGACTTCTCGGCATTGTCTCTGG43870.32751050952632293No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41930.3130274826632943No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT41630.3107878393339599No Hit
GTATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC32910.24568887322797556No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27670.2065697697422693No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG26390.19701395820377618No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26170.19537155309559767No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA25080.18723418233234965No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG24380.18200834789723622No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTATTTCCCAGGTGCC23550.17581200135274455No Hit
GTCTACTACTGCGCGAGGTCGGGAAGAGAAATTCGAACTGCTATTGGGATGTATTGGGGCCAGGGAACCGTGGTC23520.17558803701981113No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT23170.1729751198022544No Hit
AGTCAGGACATTAGCACTTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTAT22870.17073547647292012No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22650.16909307136474158No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC20820.1554312470558022No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG20450.15266902028295654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20430.1525197107276676No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG20110.1501307578430443No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGATACGGTCACCGTCTCGTGTCGGGCGAGTCAGGACATTAGC19020.14199338707979625No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCGGTCTTCAGCCTGAAGATT18950.14147080363628492No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA18480.13796202908699448No Hit
GAATGATATTGTTGGCAGTAATAAGATGCGAAATCTTCAGGCTGAAGACCGCTGATGGTGAGAGTGAAATCTGTC18420.1375141004211276No Hit
GTCCCAGACCCACTGCCACTGAACCTTCGTGGGACCCCACTTTGCAAATTGACTGCAGTATAGATCAGGGACTT18310.13669289786703837No Hit
GTGCAGCCACAGTTCGTTTGAGGTCCACTGCGGTCCCAGGGCCGAAAGTCAATGGATAAGAATGATATTGTTGGC18140.13542376664708222No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17160.12810759843792344No Hit
ATATCATTCTTATCCATTGACTTTCGGCCCTGGGACCGCAGTGGACCTCAAACGAACTGTGGCTGCACCAT17040.1272117411061897No Hit
TCTTATTACTGCCAACAATATCATTCTTATCCATTGACTTTCGGCCCTGGGACCGCAGTGGACCTCAAACGA16900.12616657421916702No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16690.124598823888633No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16390.12235918055929866No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16320.12183659711578732No Hit
ATACTGCAGTCAATTTGCAAAGTGGGGTCCCACGAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTC16200.12094073978405359No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16190.12086608500640911No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC15900.11870109645471927No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15860.11840247734414135No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15590.11638679834774046No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC15540.11601352445951808No Hit
TATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGAT15530.11593886968187359No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG15520.11586421490422912No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGGTCCACTGCGGTCCCAGGGCCGAAAGTCAATGGATAAGA15520.11586421490422912No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATAATAATTGAAGCTGAATCCAGACGTTGCAC15380.11481904801720644No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15290.11414715501840614No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15260.1139231906854727No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15260.1139231906854727No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT15250.11384853590782823No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15130.11295267857609449No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCAC15060.11243009513258315No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14950.1116088925784939No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14890.11116096391262703No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14790.11041441613618226No Hit
GTCTCGTGTCGGGCGAGTCAGGACATTAGCACTTATTTAGCCTGGTTTCA14350.10712960591982525No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTATTTCCCAGGTGCCAGATGTGACATCC14190.1059351294775136No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC13970.1042927243693351No Hit
CACCATCAGCGGTCTTCAGCCTGAAGATTTCGCATCTTATTACTGCCAACAATATCATTCTTATCCATTGACTTT13830.10324755748231242No Hit
GATTTCACTCTCACCATCAGCGGTCTTCAGCCTGAAGATTTCGCATCTTATTACTGCCAACAATATCATTCTTAT13790.1029489383717345No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTATTTCCCAGGTGCCAGATGTGACAT13750.1026503192611566No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13690.10220239059528972No Hit
GTAATAAGATGCGAAATCTTCAGGCTGAAGACCGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCC13680.10212773581764525No Hit
GTCTTCAGCCTGAAGATTTCGCATCTTATTACTGCCAACAATATCATTCTTATCCATTGACTTTCGGCCCTGGG13540.10108256893062258No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC13510.10085860459768915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)