FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339499
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATACTGCAGTCAATTTGCAAAGTGGGGTCCCACGAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTC42750.3191491744301414No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39060.29160156147932925No Hit
GTAGTAGACAGCCGTGTCCTCGCCTCTCAGGTCGTTCAATTGCAACCACACTGACTTCTCGGCATTGTCTCTGG38030.28391211938194805No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT36000.26875719952011906No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTATTTCCCAGGTGCC28490.21269146150911647No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27730.2070176984081362No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG24480.18275489567368097No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA24390.18208300267488067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23730.17715578735034515No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22550.16834652358829683No Hit
AGTCAGGACATTAGCACTTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTAT21930.1637179273743392No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT21440.1600598432697598No Hit
GTATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC21300.15901467638273714No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGATACGGTCACCGTCTCGTGTCGGGCGAGTCAGGACATTAGC20500.15304229417117893No Hit
GTCTACTACTGCGCGAGGTCGGGAAGAGAAATTCGAACTGCTATTGGGATGTATTGGGGCCAGGGAACCGTGGTC20390.15222109161708966No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC18990.14176942274686283No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18620.13900719597401714No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCGGTCTTCAGCCTGAAGATT18550.1384846125305058No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG18250.1362449692011715No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18100.13512514753650431No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC17840.1331841233177479No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG17740.13243757554130312No Hit
GTGCAGCCACAGTTCGTTTGAGGTCCACTGCGGTCCCAGGGCCGAAAGTCAATGGATAAGAATGATATTGTTGGC17490.13057120610019118No Hit
GAATGATATTGTTGGCAGTAATAAGATGCGAAATCTTCAGGCTGAAGACCGCTGATGGTGAGAGTGAAATCTGTC17160.12810759843792344No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACAC16870.12594260988623357No Hit
ATATCATTCTTATCCATTGACTTTCGGCCCTGGGACCGCAGTGGACCTCAAACGAACTGTGGCTGCACCAT16510.1232550378910324No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16340.12198590667107628No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16220.12109004933934255No Hit
GTCCCAGACCCACTGCCACTGAACCTTCGTGGGACCCCACTTTGCAAATTGACTGCAGTATAGATCAGGGACTT15990.11937298945351957No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15920.11885040601000822No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15900.11870109645471927No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGA15800.11795454867827448No Hit
TCTTATTACTGCCAACAATATCATTCTTATCCATTGACTTTCGGCCCTGGGACCGCAGTGGACCTCAAACGA15770.11773058434534106No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15540.11601352445951808No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT15530.11593886968187359No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTATTTCCCAGGTGCCAGATGTGACATCC15500.11571490534894016No Hit
GTCTGCATCTGTAGGAGATACGGTCACCGTCTCGTGTCGGGCGAGTCAGG15490.11564025057129569No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATAATAATTGAAGCTGAATCCAGACGTTGCAC15360.11466973846191747No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTATTTCCCAGGTGCCAGATGTGACAT15360.11466973846191747No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGGG15240.11377388113018375No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGT15100.11272871424316105No Hit
GCTCCTGGGGCTCCTGCTGCTCTATTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15030.11220613079964972No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14910.11131027346791598No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14790.11041441613618226No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14770.11026510658089331No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14470.10802546325155897No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGAC14460.1079508084739145No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCC14400.10750287980804762No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14350.10712960591982525No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG14310.10683098680924735No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTATTTCCCAGGTGCC14280.1066070224763139No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG14240.106308403365736No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTATTTCC14150.10563651036693571No Hit
TATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGAT13930.10399410525875719No Hit
GTCTCGTGTCGGGCGAGTCAGGACATTAGCACTTATTTAGCCTGGTTTCA13890.10369548614817929No Hit
ATACTGCAGTCAATTTGCAAAGTGGGGTCCCACGAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTC13750.1026503192611566No Hit
CTCCTGTGCAACGTCTGGATTCAGCTTCAATTATTATTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAAGGG13540.10108256893062258No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13430.10026136637653331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)