FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449199
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32910.22709096542296814No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32010.22088063820082682No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTCTGTCTCCTGGT31990.22074263092922367No Hit
GTATATGAGTTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGGTGCC31460.21708543823174042No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30270.20887400557135358No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29570.2040437510652436No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCGAGTGCTGCGGCTTGTATATGAGTTG28920.19955851473814154No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG28900.19942050746653844No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCA28520.19679836930607875No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCGAGTGCTGCGGCTTGTATATGAGTTGCAGTAATAAT28390.1959013220406583No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC25650.17699432583102803No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA25580.17651130038041704No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC25460.1756832567507982No Hit
CTCATATACAAGCCGCAGCACTCGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC25210.17395816585575893No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT24260.16740282045460975No Hit
GTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAATCCCTGAGGGCCGATT23870.16471167865834851No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG23400.1614685077756747No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23310.1608474750534606No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTCTGTCTCCTGGTACCAACACCACCCAGGCAAAGC22290.15380910420170038No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21960.15153198422024858No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAACTCATAT21910.1511869660412407No Hit
GTGTTGGTACCAGGAGACAGAGTCATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG21510.1484268206091779No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21350.14732276243635278No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC20590.14207848611543342No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20400.1407674170352036No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT19730.13614417343649837No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG19320.13331502436863396No Hit
CAGTAATACAAGGCGGTGTCTTCAGGTCTCAGACTGTTCATTTGCAGATA19290.13310801346122927No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC19120.13193495165260258No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTCTGTCTCCTGGTACCAACACC18800.1297268353069523No Hit
GTCTGGCACCACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACG18320.12641466078847696No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC18100.1248965808008424No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17950.12386152626381883No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17850.12317148990580315No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT17520.12089436992435132No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG17420.12020433356633561No Hit
CCCGAGTGCTGCGGCTTGTATATGAGTTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTC17040.11758219540587593No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16880.11647813723305081No Hit
GATTATTACTGCAACTCATATACAAGCCGCAGCACTCGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGT16860.11634012996144767No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16520.11399400634419428No Hit
GTAATACAAGGCGGTGTCTTCAGGTCTCAGACTGTTCATTTGCAGATACAAGGAGTTTTTGCTGTTGTCTCTGG16410.11323496635037701No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG16240.11206190454175032No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16180.11164788272694087No Hit
GTATTACTGTGCCGCGTGTTATAACAGCAGCTGGTGTGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC16080.1109578463689252No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC16030.11061282818991733No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC16010.11047482091831419No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGGTGCCAGACTTGGAGCCAGAGAAG16000.11040581728251261No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAGTGGA15930.10992279183190162No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15790.10895674093067964No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT15710.10840471184426707No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15280.10543755550479954No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC15060.103919475517165No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14840.10240139552953045No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14700.10143534462830847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)