FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1449199
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33500.23116217993526078No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31430.2168784273243357No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCGAGTGCTGCGGCTTGTATATGAGTTGCAGTAATAAT31270.21577436915151058No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCTGCCGAACACCCGAGTGCTGCGGCTTGTATATGAGTTG30910.21329023826265406No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTCTGTCTCCTGGT30430.20997806374417868No Hit
GTATATGAGTTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGGTGCC29450.20321570743562478No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGC27420.18920796936790601No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26710.18430871122599451No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC26200.1807895258001144No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA26150.18044450762110656No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG26100.18009948944209872No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25360.1749932203927825No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCA24440.16864488589903803No Hit
CTCATATACAAGCCGCAGCACTCGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGC23930.16512570047315792No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCT23190.16001943142384173No Hit
GTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAATCCCTGAGGGCCGATT23070.15919138779422287No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21610.14911685696719362No Hit
GTGTTGGTACCAGGAGACAGAGTCATAACCACCAATGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG20290.1400083770413863No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTCTGTCTCCTGGTACCAACACCACCCAGGCAAAGC20000.13800727160314077No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19890.13724823160932348No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGC19870.13711022433772035No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19550.1349021079920701No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGAT19420.1340050607266497No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAACTCATAT19380.13372904618344342No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19200.13248698073901516No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18500.12765672623290522No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCG18220.12572462443046123No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTC18000.12420654444282668No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17760.12255045718358899No Hit
CAGTAATACAAGGCGGTGTCTTCAGGTCTCAGACTGTTCATTTGCAGATA17610.12151540264656545No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC17530.12096337356015288No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG17450.12041134447374033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17190.1186172499428995No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16590.11447703179480527No Hit
GTCTGGCACCACGGCCTCCCTGACCATCTCTGGGCTCCAGGCTGAGGACG16580.1144080281590037No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC16410.11323496635037701No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCTGAACCTGCGGGAGTCGGCCACCATC16140.1113718681837346No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGG16060.11081983909732203No Hit
GATTATTACTGCAACTCATATACAAGCCGCAGCACTCGGGTGTTCGGCAGAGGGACCAAGCTGACCGTCCTAGGT15950.11006079910350476No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTCTGTCTCCTGGTACCAACACC15850.10937076274548906No Hit
CCCGAGTGCTGCGGCTTGTATATGAGTTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTC15800.1090257445664812No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15700.10833570820846551No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15540.10723165003564036No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15480.10681762822083096No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG15470.10674862458502937No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15380.10612759186281524No Hit
GTAATACAAGGCGGTGTCTTCAGGTCTCAGACTGTTCATTTGCAGATACAAGGAGTTTTTGCTGTTGTCTCTGG15270.10536855186899798No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15050.10385047188136343No Hit
GTATTACTGTGCCGCGTGTTATAACAGCAGCTGGTGTGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC14610.10081431190609434No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC14590.1006763046344912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)