FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081722
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTCTCAGGGAGA32430.2997997637100845No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24580.2272302865246339No Hit
GTCTCTGGTGACTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGAT23600.2181706575256859No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC22870.2114221583734083No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT22420.20726212464940158No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC19140.17694010106108593No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19120.1767552106733523No Hit
GTATGGTAGGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18740.17324229330641328No Hit
ATCTATGACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGGGTCACCATATCAGTAGACACGTCCA18620.1721329509800115No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGG17840.16492222585839986No Hit
GTGTATTACTGTGCGAGACTTGGGGAGCGGTGGCTGGCCCTTGGGTACTTCGACCCCTGGGGCCAGGGAACCCT17790.1644599998890658No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17620.16288843159332989No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17600.16270354120559627No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17570.16242620562399582No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16480.15234967949251285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16370.1513327823599779No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTCTCAGGGAGAACTGGGTCTTGGACGTGTCT16370.1513327823599779No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16370.1513327823599779No Hit
GGCTATATCTATGACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG15910.14708030344210435No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG15710.14523139956476802No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15580.14402961204449943No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC15570.1439371668506326No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC15500.14329005049356489No Hit
CTATTACTGTCAGCAGTATGGTAGGTCACCGTGGACGTTCGGCCAAGGGA15340.14181092739169585No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14850.13728111289222183No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC13760.1272045867607389No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC13740.12701969637300528No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGG13350.12341433381219945No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13200.1220276559041972No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT13140.12147298474099631No Hit
GTCTATTACTGTCAGCAGTATGGTAGGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA13120.12128809435326268No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC12990.12008630683299407No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT12880.11906940970045907No Hit
GTCATAGATATAGCCTATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA12690.11731295101698959No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGC12600.11648094427218823No Hit
CCCTGAGACTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGACTTGGGGAGCGGTGGCTGG12540.11592627310898734No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12410.11472448558871873No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12400.1146320403948519No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12230.11306047209911603No Hit
GTCTCAGGGAGAACTGGGTCTTGGACGTGTCTACTGATATGGTGACCCGA12160.11241335574204833No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12140.1122284653543147No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGTGACCTACCATACTGCTGAC12100.11185868457884744No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12100.11185868457884744No Hit
CTACCATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11900.11000978070151111No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11880.10982489031377748No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11850.10954755473217702No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG11700.1081608768241748No Hit
GTATTACTGTGCGAGACTTGGGGAGCGGTGGCTGGCCCTTGGGTACTTCGACCCCTGGGGCCAGGGAACCCTAGT11670.10788354124257434No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC11630.10751376046710708No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11630.10751376046710708No Hit
CCATATCAGTAGACACGTCCAAGACCCAGTTCTCCCTGAGACTGAGCTCTGTGACCGCCGCAGACACGGCCGTGT11490.10621952775297167No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11450.10584974697750438No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11390.10529507581430349No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTG11380.10520263062043667No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11330.1047404046511026No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11250.10400084310016805No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCAC11200.10353861713083398No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACTAGGGTTCCCTGGCCCCAGGGGTCGAAGTACCCAAGGG11110.10270661038603264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA11060.10224438441669854No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10970.10141237767189722No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10900.1007652613148295No Hit
CTTTCTGAGAGTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCC10870.10048792573322907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)