FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081722
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTCTCAGGGAGA29430.2720662055500397No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24960.23074320389157285No Hit
GTCTCTGGTGACTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGAT21890.20236252937446036No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACC20750.1918237772736433No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGT19840.18341126463176308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19130.17684765586721912No Hit
ATCTATGACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGGGTCACCATATCAGTAGACACGTCCA18300.1691747047762734No Hit
GTGTATTACTGTGCGAGACTTGGGGAGCGGTGGCTGGCCCTTGGGTACTTCGACCCCTGGGGCCAGGGAACCCT18050.166863574929603No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18020.16658623934800254No Hit
GTATGGTAGGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT17540.16214887004239537No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGG15970.14763497460530525No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15720.14532384475863483No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15660.14476917359543395No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15540.14365983126903217No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15460.14292026971809763No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTCTCAGGGAGAACTGGGTCTTGGACGTGTCT15440.14273537933036398No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15380.1421807081671631No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC14990.1385753456063573No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGC14980.13848290041249045No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14630.1352473186271519No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14590.13487753785168463No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14520.13423042149461692No Hit
CTATTACTGTCAGCAGTATGGTAGGTCACCGTGGACGTTCGGCCAAGGGA14410.13321352436208195No Hit
GGCTATATCTATGACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG13930.12877615505647477No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT13890.12840637428100748No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13780.12738947714847254No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13460.1244312309447344No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAA13420.12406145016926715No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG13360.12350677900606626No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13310.12304455303673219No Hit
GTCTCAGGGAGAACTGGGTCTTGGACGTGTCTACTGATATGGTGACCCGA13140.12147298474099631No Hit
GTCTATTACTGTCAGCAGTATGGTAGGTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA13060.12073342319006176No Hit
GTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC13000.12017875202686087No Hit
GTCATAGATATAGCCTATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA12970.11990141644526042No Hit
CAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC12870.11897696450659226No Hit
CTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGC12530.11583382791512052No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC12300.11370758845618376No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12230.11306047209911603No Hit
CCCTGAGACTGAGCTCTGTGACCGCCGCAGACACGGCCGTGTATTACTGTGCGAGACTTGGGGAGCGGTGGCTGG12040.11130401341564652No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11780.10890043837510932No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGTGACCTACCATACTGCTGAC11670.10788354124257434No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG11570.10695908930390619No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTT11410.10547996620203712No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGTGACTAGGGTTCCCTGGCCCCAGGGGTCGAAGTACCCAAGGG11410.10547996620203712No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11350.10492529503883623No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11330.1047404046511026No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG11170.10326128154923353No Hit
CTACCATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11110.10270661038603264No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11110.10270661038603264No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11030.1019670488350981No Hit
GTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA10970.10141237767189722No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCC10910.10085770650869633No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)