FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498882
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC13250.2655938678885989No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT10980.22009212599372197No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT10380.20806523386291748No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA10050.201450443190975No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT9990.20024775397789457No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA8260.16557021500074168No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC6950.1393115005151519No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGA6680.13389939905628986No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT6680.13389939905628986No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT6580.13189491703448913No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6540.13109312422576883No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTG6400.1282868493952478No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA6380.12788595299088762No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC6310.1264828155756271No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA6300.12628236737344703No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA6160.12347609254292598No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCCGAGCAGT5990.1200684731058647No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGA5740.11505726805136283No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACT5200.10423306513363882No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT5200.10423306513363882No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC5190.10403261693145874No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGG5150.10323082412273844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)