FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004717903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869029
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT22090.2541917473409978No Hit
GTAATAGACGGCCGTGTCGTCAGCTCTCAGGCTGTTCATTTGAAGATACAGGGTGTTCTCGGAATCGTGTCTGG22030.2535013215899585No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19150.22036088554006827No Hit
CTCCTACACTTATGTCTTCGGAACTGGAACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTC18930.21782932445292388No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17490.20125910642797884No Hit
GTGTAGGAGCCTGCATATGAGCAGCAGTAATACTCAGCCTCATCCTCAGC16800.19331921029102594No Hit
GTATTACTGCTGCTCATATGCAGGCTCCTACACTTATGTCTTCGGAACTGGAACCAAGGTCACCGTCCTAGGTC16360.1882560881167372No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16330.1879108752412175No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC16080.18503410127855344No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG15970.18376832073498123No Hit
GAGTATTACTGCTGCTCATATGCAGGCTCCTACACTTATGTCTTCGGAACTGGAACCAAGGTCACCGTCCTAGGT15500.17835998568517278No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGAGATGTTGGTAATAATAACTATATCTCCTGGT15270.1757133536395218No Hit
GTCTATTACTGTGCGAGAGATGCCGGGTATAGAGTCTACTGGGGCCAGGG14530.16719810271003613No Hit
ACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTGTAGGAGCCTGCATATGAGCAGCAGTAATACTC14460.16639260600049022No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14390.16558710929094428No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC14290.16443639970587862No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC14200.16340076107931956No Hit
GACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTGTAGGAGCCTGCATATGAGCAGCAGTAATACT14090.16213498053574738No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14070.16190483861873425No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13350.1536197296062617No Hit
ATACTACACAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGACACGATT12930.14878674934898606No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGAGTATTACTGCTGCTCATAT12280.14130713704605946No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12030.13843036308339537No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG11680.13440287953566568No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11630.13382752474313286No Hit
CTCCTGCACTGGAACCAGCAGAGATGTTGGTAATAATAACTATATCTCCTGGTACCAACAACACCCAGGCAACGC11550.13290695707508035No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTGTAGGAGCCTGCATATGAGC11460.13187131844852126No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT10910.1255424157306603No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10900.12542734477215375No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC10890.12531227381364718No Hit
CTATATCTCCTGGTACCAACAACACCCAGGCAACGCCCCCAAATTCATGATTTATGATGTCAGCAAGCGGCCCTC10840.12473691902111436No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG10490.12070943547338465No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10200.11737237767669433No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10030.11541617138208277No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT9960.11461067467253683No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGAGATGTTGGTAATAATAACTATATCTCCTGGTACCAACAAC9920.11415039083851057No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT9880.11369010700448433No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTGTAGG9830.1131147522119515No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9620.11069826208331367No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9500.10931741058123493No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9430.10851191387168899No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAGAGGTGTCC9310.10713106236961022No Hit
GTGTAGTATGTCCTACCGTCGCTATAAAAAATTGAGACCCATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCA9280.10678584949409053No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC9090.10459950128246583No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9050.10413921744843958No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG9000.10356386265590677No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG8870.10206794019532145No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8790.10114737252726895No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG8760.10080215965174925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)