FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004717904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004717904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869029
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23010.2647782755236016No Hit
GTAATAGACGGCCGTGTCGTCAGCTCTCAGGCTGTTCATTTGAAGATACAGGGTGTTCTCGGAATCGTGTCTGG20410.23485982631189523No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18880.21725396966039107No Hit
CTCCTACACTTATGTCTTCGGAACTGGAACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTC17820.20505644805869538No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17740.2041358803906429No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC17310.1991878291748607No Hit
GTGTAGGAGCCTGCATATGAGCAGCAGTAATACTCAGCCTCATCCTCAGC16080.18503410127855344No Hit
GTATTACTGCTGCTCATATGCAGGCTCCTACACTTATGTCTTCGGAACTGGAACCAAGGTCACCGTCCTAGGTC15650.1800860500627712No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGAGATGTTGGTAATAATAACTATATCTCCTGGT15550.1789353404777056No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC15130.1741023602204299No Hit
GAGTATTACTGCTGCTCATATGCAGGCTCCTACACTTATGTCTTCGGAACTGGAACCAAGGTCACCGTCCTAGGT14780.17007487667270021No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14170.1630555482037999No Hit
GACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTGTAGGAGCCTGCATATGAGCAGCAGTAATACT14120.16248019341126707No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG14010.16121441286769486No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC13290.15292930385522233No Hit
GTCTATTACTGTGCGAGAGATGCCGGGTATAGAGTCTACTGGGGCCAGGG13240.1523539490626895No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13090.15062788468509106No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTC12220.14061671129502007No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGAGTATTACTGCTGCTCATAT11970.137739937332356No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGT11840.13624401487177068No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTGTAGGAGCCTGCATATGAGC11790.13566866007923786No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG11710.13474809241118535No Hit
ACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTGTAGGAGCCTGCATATGAGCAGCAGTAATACTC11410.13129596365598847No Hit
CTCCTGCACTGGAACCAGCAGAGATGTTGGTAATAATAACTATATCTCCTGGTACCAACAACACCCAGGCAACGC11230.12922468640287033No Hit
ATACTACACAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGACACGATT11080.1274986220252719No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11080.1274986220252719No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10720.12335606751903562No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10380.11944365492981247No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTC10220.11760251959370746No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9800.1127695393364318No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9710.11173390070987274No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9540.10977769441526117No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC9530.10966262345675461No Hit
CTATATCTCCTGGTACCAACAACACCCAGGCAACGCCCCCAAATTCATGATTTATGATGTCAGCAAGCGGCCCTC9450.10874205578870211No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9430.10851191387168899No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCCCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG9290.1069009204525971No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9210.1059803527845446No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTGTAGG9090.10459950128246583No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9070.1043693593654527No Hit
GTGTAGTATGTCCTACCGTCGCTATAAAAAATTGAGACCCATTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCA9050.10413921744843958No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAGAGGTGTCC9040.10402414648993301No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9020.10379400457291989No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGG8950.10298850786337393No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT8940.10287343690486739No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGAGATGTTGGTAATAATAACTATATCTCCTGGTACCAACAAC8900.10241315307084115No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG8780.10103230156876238No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)